666 / 670
Table 1: Accuracy—Recovery
Average
RSD
Average
RSD (%)
Child Formula Powder
00847RF00
3
1.6 (IU hg
-1
)
135 (IU hg
-1
)
97% 285 (IU hg
-1
)
101%
Infant Elemental Powder
00796RF
3
2.4 (IU hg
-1
)
136 (IU hg-1)
98% 276 (IU hg
-1
)
98%
Adult Nutritional RTF High Protein
00821RF00
3
0.5 (IU L
-1
)
269 (IU L
-1
)
98% 555 (IU L
-1
)
99%
Adult Nutritional RTF High Fat
00820RF00
3
3.6 (IU L
-1
)
270 (IU L
-1
)
98% 559 (IU L
-1
)
99%
Infant Formula RTF, Milk-Based
EV4H2Q
3
3.0 (IU L
-1
)
268 (IU L
-1
)
97% 547 (IU L
-1
)
97%
Child Formula Powder
00847RF00
3
0 (IU hg
-1
)
198 (IU hg
-1
)
100% 408 (IU hg
-1
)
101%
Infant Elemental Powder
00796RF
3
0 (IU hg
-1
)
191 (IU hg-1)
96% 395 (IU hg
-1
)
98%
Adult Nutritional RTF High Protein
00821RF00
3
0 (IU L
-1
)
389 (IU L
-1
)
98% 789 (IU L
-1
)
98%
Adult Nutritional RTF High Fat
00820RF00
3
0 (IU L
-1
)
403 (IU L
-1
)
101% 813 (IU L
-1
)
101%
Infant Formula RTF, Milk-Based
EV4H2Q
3
0 (IU L
-1
)
375 (IU L
-1
)
94% 789 (IU L
-1
)
98%
Global Mean
98%
99%
yes
Were products weighed into extraction vessels before spiking?
Were all spike recovery samples prepared on the same day?
+ Spike Level 2
Spike Recovery
Product
Replicates
(n)
Native level
Were products spiked before they were weighed into extraction vessels?
Analyte
no
no
+ Spike Level 1
SPIFAN Code
Was a portion of the product spiked?
Were all spike recovery samples taken from the same spiked product?
Were multiple portions of the product spiked and each spiked product analyzed?
Vitamin D
2
Vitamin D
3
Additional information to be completed by SD
yes
no
yes
What percentage of the spiked sample was the spiked was the spike blanc? (How much was product diluted by spike?)
Ethanol
0.5% (50 μL in 10 mL)
vitamin D
2
and vitamin D
3
Were all spike recovery samples prepared on multiple days?
Were spike recoveries calculated from theoretical additions?
Or was spiking solution analyzed and the results used to calculate spike recoveries?
yes
no
yes
What form of the nutrient was spiked into the product?
What kind of solution or solvent was used to deliver the spike to the product?
SPIFAN ERP Checklist v1.0
VitD-18 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE