SPIFAN Meeting (September 17, 2016)

AOAC Official methods

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AOAC 961.16 (Titrimetry)

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AOAC 944.08 (Ash-Distillation-Titration)

These 2 methods are not suitable for determining low levels of fluoride in infant

formula and adult nutritional products.

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Microdiffusion and F-ISE (

Microdiffusion and fluoride-specific electrode

determination of fluoride in infant foods: collaborative study

- Dabeka, R. W.,

McKenzie, A. D. J. AOAC., 64, (4): 1021-1026,1981)

Results from collaborative study were not accepted by AOAC. Method not

published.

September 2016

German Official Method (Fluo-01)

Amtliche Sammlung von Untersuchungsverfahren, nach Paragraph 35 LMBG.(L 49.00-

7): 2pp., 2000 -

Analysis of foods. Determination of fluoride in dietetic foods with an

ion-sensitive electrode.

•

Only validated on 2 dietetic products

•

Modified and validated with 30 Healthcare products (Nestlé method):

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PLOQ RTF: ≈50 μg/100g (Dilution factor 2 with TISAB)

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Good RSDr (< 5%) / RSDiR (< 8%) / Recovery (83%-109%)*

*Bad Recovery for products with high protein/thickeners/caseinates due to non

specific binding of measured free fluorides

Conclusion: method not sensitive & not applicable to products with high protein /

thickener/caseinates content + SLV (SPIFAN kit)

September 2016