SPIFAN Meeting (September 17, 2016)

Ion Chromatography Method (Fluo-02)

ThermoFisher Scientific. Analysis of fluoride in Infant Formula and Adult Nutritionals by

ion chromatography after ultracentrifugation

•

Good linearity (> 0.995) and estimated LOQ (≈ 5μg/100g)

•

Good RSDr (< 5%) , RSDR (<10%) and recovery (81%-105%) for 12 SPIFAN products

•

Specific equipment and IC consumables (column, cartridges, buffer) needed /

Chloride analysed simultaneously

Conclusion: equipment dependent / method to be improved for low F level (e.g.

removal of organic acids, column and buffer used, sample volume injected) for a better

chromatogram interpretation / Complete SLV & additional SLV chloride could be a plus

using improved method

September 2016

Microwave acid digestion and F-ISE

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Alternative to tedious & time consuming alkali fusion or microdiffusion

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LOQ blank: 5 μg/L / LOQsample ≈ 20 μg/100g dry matter (1g with DF=2 TISAB 20%) /

20 food samples (seafood, vegetables, fruits: 0.08 – 1.8 mg/100g dry matter)

•

Not validated for infant formula: (estimated LOQ :< 20 μg/100g RTF)

Conclusion: feasibility to test for Infant Formula with some modifications (e.g. diluted

HNO

/ 2g IF reconstituted / F-ISE calibration type)

September 2016

Reference 10 is listed

in slide 16