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Methods (FDA)
FDA Method
Spectra and Q/Confirmation Ions of Powdered Kratom Leaf
1200000
1400000
1600000
1800000
2000000
Abundance
Scan 1706 (14.649 min): 07071410.D
397
214
50 100 150 200 250 300 350 400 450 500 550 600 650
0
200000
400000
600000
800000
1000000
m/z-->
269
186
367
75
155
327
129
240 295
103
429 479 553
505
589619647
Challenges
•
GC Methods
–
not commonly applied for
M. speciosa
, particularly in the wide
variety of matrices encountered
–
High temperatures required to elute the alkaloids – restricts
parameter adjustment for resolution of any alkaloid mixture
–
inadequate resolution of mitragynine and speciociliatine
–
Reliance on mass‐specific detection – EI, ESI, and ESI MS/MS
spectra of mitragynine, speciogynine, and speciociliatine are
nearly identical
–
Interference of speciociliatine – would probably not be detected
by either DAD or MS detectors
–
Methods should account for interference
–
derivatization may be necessary to overcome poor chrom. res.