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Submission Date
2016-09-15 11:05:32
Name
Rachel Stryffeler
Organization
The Coca-Cola Company
Title of Method
Determination of Alcohol Content in Kombucha Tea by Headspace Solid Phase
AOAC Candidate Method
Number (e.g. ALN-01)
KOM-05
Applicable SMPR
Standard Method Performance Requirements (SMPRs®) for Determination of Ethanol
in Kombucha
Summary:
This method uses the headspace analysis ethanol by gas chromatography-mass
spectrometry (GC-MS) for quantitation of ethanol in kombucha. Ethanol in the
headspace is extracted by solid phase micro-extraction (SPME) followed by separation
and detection by GC-MS.
1. Does the applicability of
the method support the
applicability of the SMPR? If not,
please explain what is missing.
GC-MS is a suitable technique for the analysis of trace volatile compounds such as
ethanol. The method was applied successfully to the quantitation of ethanol in
kombucha.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
For a significant portion of the method validation and testing, non-kombucha teas were
used for the analysis. A tea blank was prepared with tea and sugar, but it was not
fermented and is assumed to be a still beverage. This may impact the results. Actual
kombucha tea samples were tested in duplicate, but not used for a significant portion of
the method performance testing.
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
There were no precautions or warnings given in the method. Safety warnings may be
recommended for select reagents and solvents.
AOAC SPSFAM ERP REVIEW: MAIN FORM
I. Summary of Method
II. Review of Method Only