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Format for AOAC
Official Methods of
Analysis
Online Technical Resources
Method Development, Optimization & Validation
™ OMA Ͳ Appendix F Ͳ Guidelines for Standard
Method Performance Requirements
™ Homogeneity
™ Guide for Writing Methods in AOAC Format
™ Statistics Protocol Review Form
™ OMA Ͳ Appendix D: Guidelines for Collaborative
Study Procedures to Validate Characteristics of a
Method of Analysis
™ OMA Ͳ Appendix G: Procedures and Guidelines for
the Use of AOAC Voluntary Consensus Standards to
Evaluate Characteristics of a Method of Analysis
™ OMA Ͳ Appendix I: AOAC INTERNATIONAL Methods
Committee Guidelines for Validation of Biological
Threat Agent
™ Methods and/or Procedures
™ OMA Ͳ Appendix J: AOAC INTERNATIONAL Methods
Committee Guidelines for Validation of
Microbiological Methods for Food and
Environmental Surfaces
™ OMA Ͳ Appendix K: Guidelines for Dietary
Supplements and Botanicals
™ OMA Ͳ Appendix L: AOAC Recommended
Guidelines for Stakeholder Panel on Infant Formula
and Adult Nutritionals (SPIFAN) SingleͲLaboratory
Validation
™ OMA Ͳ Appendix M Ͳ Validation Procedures for
Quantitative Food Allergen ELISA Methods:
Community Guidance and Best Practices
™ Safety Checklist
Method Review
™ Examples of Statistical Analysis
™ Statistics Manuscript Review Form
™ OMA Ͳ Appendix A: Standard Solutions and
Reference Materials
™ OMA Ͳ Appendix D: Guidelines for Collaborative
Study Procedures to Validate Characteristics of a
Method of Analysis
™ OMA Ͳ Appendix H: Probability of Detection (POD)
as a StatisticalModel for the Validation of
Qualitative Methods
Miscellaneous
™ Definition of Terms and Explanatory Notes
™ OMA Ͳ Appendix B: Laboratory Safety
™ OMA Ͳ Appendix E: Laboratory Quality Assurance
™ OMA Ͳ Appendix C: Reference Tables
All resources are accessible at
Forquestions,please contact:
P301-924-7077 x157 E
Thelanguageofthemethodshouldbeconciseandcompletelyfreefromambiguity.
Concisenessisdesirable,bothtoensureclarityandtosavespace. Wheneverthereisaconflict
betweenclarityandstyle,clarityismoreimportant.
PresentTenseandImperativeMode
Checksentencesthatdonotbeginwithaverbandchangethem,iffeasible,tothe
imperativemode(e.g.Pipet10mL...,Stir...,etc.).Exceptionsare:useofadverbmodifier
("Accuratelyweigh..."),prepositionalclause("Forrefinedsugars,use..."),permissive
statements("Ferrichydroxidemaybeused..."),andstatementsinthe"Principle"section.
Abbreviations
MostabbreviationsarethesameasthoseusedbyChemicalAbstracts.Donotuse
abbreviationsintitlesandheadings. Seethe DefinitionsofTermsandExplanatoryNotes.
RepetitionandRedundancy
Eliminaterepetitionandredundancyasfaraspossible;useonlyforemphasis.Donotuse
"distilled"withwater,"concentrated"withcommonacids,"95%"withalcohol,or"ACS"with
reagentscoveredbyACSspecifications.Theseareunderstoodbydefinition.
Terminology, FormulaeandChemicalNames
Fornamesofchemicalcompounds,usethespelling,hyphenation,andworddivisiongivenin
ChemicalAbstracts. Useanationalpharmacopeiafornamesfordrugs. UseISO
nomenclatureforpesticidesandCodexnomenclaturefornamesoffoodadditivesandcolor
additives.
Consistency
Watchforinternalcontradictionsinthetext:volumesthatdonotadduporthatexceedthe
capacityofthecontainer;tooabruptatransitionfromoneoperationtoanother(alinemay
beomitted);andimpracticalorimpossiblenumbers(e.g.,100gNaCl willnotdissolvein100
mLwater).
CrossͲreferences
AllnewAOACmethodsshouldbewrittenascompleteandselfͲcontainedaspractical. Do
notrefertootherAOACmethods. IfpartofaprocedureinanOfficial MethodSMistaken
frommaterialpreviouslypublishedelsewhere,incorporatethosestepsinthemethodrather
thanreferringtheanalysttoanotherpublication.
Definitions
Thesection"DefinitionofTermsandExplanatoryNotes,"Official MethodsofAnalysisof
AOACINTERNATIONAL,isthebasicguidetoconventionsandconsistency.
IllustrationsandTables
Ifsymbolsareusedonthefigure,includeanexplanationinthecaptionortext. Provide
descriptivetitlesfortables. Explainanyobscureheadingsinafootnote.
BibliographicReferences
Checkallreferencesforaccuracy. UsestandardChemicalAbstractsabbreviationsfor
Journaltitles. Ingeneralavoidreferencesinmethod. Citebackgroundreferencesinthe
"Introduction"or"Discussion"sectionofthecollaborativestudymanuscriptͲͲnotinthe
method. Ifpartofaprocedureinan Official MethodSMistakenfrommaterialpreviously
publishedelsewhere,incorporatethosestepsinthemethodratherthanreferringthe
analysttoanotherpublication.
Safety
All methodsmustbereviewedforsafetyandpotentialhazards. Methodsshould
automaticallyincorporatecrossͲreferencestothesafetystatement(s),orpresent
questionedconditionstotheattentionoftheCommitteeonSafetyforresolution.
Decisionsregardinginclusionofsafetystatementsshouldbepractical,recognizingthat
overusewillbeselfͲdefeating.
Methodsthatcreatetoxic,obnoxiousorenvironmentallyhazardousfumesandwastes
shouldcontainpracticaldirectionsfordisposal.
CheckingEditedCopyandProofreading
Theauthormustreviewacopyoftheoriginalversionandeditedcopytoensurethatthere
hasbeennochangeinmeaning,tocorrecttypographicalerrors,andtoanswerany
questionsposedbytheeditor. Theauthormustreviewthetypesetmethodforaccuracy.
RevisedOctober2013
©2013CopyrightAOACINTERNATIONAL
Insert Headline Here
TheAOACstyleusedforpreparingmethodsforpublicationinthe Official Methodsof
AnalysisofAOACINTERNATIONAL includesthefollowingessentials:
9 Standardizedformatthatfollows theorderoflaboratoryoperations.
9 Useoftheimperativemode.
9 CrossͲreferencestoidenticalreagents,apparatus,andoperations.
9 Useofstandardizeddefinitions,terminology,andstyle.
9 Useofacceptedabbreviationsandsimplifications.
9 UseofSIunits
9 MethodsshouldbewrittenascompleteandselfͲcontainedaspractical.
9 NormalityshouldbereferredintermsofMolarity.
9 ppmshould bechangedtomg/kgormg/L
9 ppbshould bechangedtong/gorng/mL
9 pptshould bechangedtopg/gorpg/mL
FORMAT OF AOAC® OFFICIAL METHODS
of ANALYSIS OF AOAC INTERNATIONAL
Title:
™ Includesanalytebeingdetermined,typeof
matrix(matrices),andanalyticaltechnique
usedforanalysis.
Applicability:
™ Includeslistofmatrix(es)alongwithspecific
matrixtypesandrangeorlimitsof
determinationordetection.
Precautions:
™ Makesananalystawareofhazardous
materialsusedinanalysis.
DataCollection:
™ Table(s)thatpresentsperformance
parametersincludingmatricestestedina
collaborativestudy,levelsofanalyte(s),%
recovery,RSDr,RSD R,sr,s R,HORRAT,number
ofobservations,etc
Principle:
™ Explainsscientificpremiseonwhichthe
methodisoperatesspecificallythemechanism
oftheanalysis.
Apparatus:
™ Liststheequipmentthatrequiresassemblyor
thathasspecifications criticaltothemethod
performance.Describeequipmentintermsof
performancecharacteristics.
Reagents:
™ Listthereagentswithamountsand
appropriateunitsneededtoconductthe
analysis anddescribethereagentsintermsof
performancecharacteristics.
SampleandTestPortionPreparation:
™ Describethepreparationofsamplesandthe
testportion.
Determination:
™ Describestheactualanalysis.
Calculations:
™ Sectionthatexplainshowtocalculatefinal
results;presentedinaformofequationor
description.
Othersectionsasneeded
REFERENCING AOAC ®OFFICIAL METHODSSM
Whenreferencing AOAC®OfficialMethodsSM,only
themethodnumbershould beusedasseeninthe
followingexample:
(1) Official MethodsofAnalysisofAOACINTERNATIONAL
(2012)19thEd.,AOACINTERNATIONAL,
Gaithersburg, MD,USA,OfficialMethod2008.01
RevisedOctober2013
©2013CopyrightAOACINTERNATIONAL