23
B
6
Regulatory Information
Min
Max
US Infant Formula
0.035 mg/100kcal
0.245 mg/L*
‐‐
EU Infant formula
0.035 mg/100kcal
0.245 mg/L*
0.10 mg/100kcal
0.70 mg/L
EU FSMP
0.08 mg/100 kcal
0.56 mg/L*
0.24 mg/100kcal
1.6 mg/L
Codex Infant Formula 0.035 mg/100 kcal
0.175 mg/100kcal
0.245 mg/L*
1.225 mg/L
Aus/NZ Infant
Formula
0.038 mg/100kcal
0.266 mg/L*
0.15 mg/100kcal
1.05 mg/L
China (GB) Infant
Formula
0.035 mg/100 kcal
0.245 mg/L*
0.175 mg/100kcal
1.225 g/L
*based on 70 kcal/100mL
Current Official Testing Methods
B
1
AOAC 942.23 (Food) and 986.27 (IF) HPLC with
fluorescence detection, acid hydrolysis, enzyme
treatment, and pre‐column thiachrome
formation.
GB 5413.11, EN 14122 and BIS 5398 all employ a
very similar approach as the AOAC methods with
the EN method allowing for post‐column
thiachrome conversion, and the BIS method also
including the microbiological method option.
