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Current Official Testing Methods
B
2
AOAC 970.65 (Food) and 985.31 (IF) fluorometric
detection, acid hydrolysis, , and an oxidation step
to remove potential interferences.
GB 5413.12, EN 14152 are HPLC methods with
fluorescence detection, acid hydrolysis is
combined with enzymatic treatment.
BIS 5399 combines the AOAC approach with the
classic microbiological methodology.
Current Official Testing Methods
B
3
AOAC 985.34 (Food) Microbiological, one of the
simpler and more reliable microbiological
methods.
EN 15652 An HPLC method using fluorescence
detection, Niacin is derivatized using UV radiation.
GB 5413.15 HPLC with UV detection and
microbiological method option.
BIS 5400 Classic photometric method and
microbiological method.
