25
Current Official Testing Methods
B
6
AOAC 985.32 microbiological, acid hydrolysis, and
enzymatic treatment.
AOAC 2004.07 & EN 14164 HPLC with fluorescence
detection, acid hydrolysis, enzymatic treatment combined
with chemical transformation of all three forms of B6 to
Pyridoxine.
EN 14663 HPLC with fluorescence detection, acid
hydrolysis, enzymatic treatment all three forms of B
6
quantitated separately
What do we need to test?
Vitamin
Forms
What should we What should we
test?
report?
B
1
Thiamin (free)
TMP
TPP
TTP
Total B
1
Thiamine Chloride
Hydrochloride
B
2
Riboflavin (free)
FMN
FAD
Total B
2
Riboflavin
Nicotinic Acid
Total B
Nicotinamide
B
3
Nicotinamide
3
B
6
Pyridoxine
Pyridoxal
Pyridoxamine
PNP,PLP,PMP
Total B
6
Pyridoxine
