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Analytical Challenges
Low levels of some inherent forms of
vitamins such as nicotinic acid and
Pyridoxamine.
Assuring that the extraction procedure is
adequate for total vitamin content.
Stability! Assuring lab conditions are
adequate for the testing of labile nutrients
such as Riboflavin.
Future Direction
or
Is LC/MS the Panacea of WSV testing?
WSVs are generally a natural fit for MS ionization.
Internal standards are available for almost all WSVs.
MS solves several detection issues with some WSVs such
as Thiamine and Niacin.
However you do still need considerable sample prep in
certain cases to get total vitamin content.
Many HPLC techniques can be very reliable, precise,
accurate, and potentially less complicated to perform.