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Salmonella Methods Harmonization Working
Group Members
Thomas Hammack (Chair)
FDA - CFSAN
Patrice Arbault
Nexidia
Marcia Armstrong
QIAGEN Gmbh
Mike Clark
Bio-Rad Laboratories
Erin Crowley
Q Laboratories, Inc.
Leanne DeWinter
Health Canada
Philip Feldsine
BioControl Systems, Inc.
Paul In't Veld
Netherlands Food and Consumer Product Safety
Authority
Irene Iugovaz
Health Canada
Balamurugan Jagadeesan
Nestec S.A
Ron Johnson
bioMerieux
Adrianne Klijn
Nestle Quality Assurance Laboratory
W d L
Bi R d L b t i
I
en y auer
o- a a ora or es, nc.
Wendy McMahon
Silliker Inc.
Sam Mohajer
Canadian Food Inspection Agency
Kirsten Mooijman
Coordinator EURL-Salmonella
Mark Mozola
Neogen Corporation
Brooke Schwartz
Brooke Schwartz Consulting
Meredith Sutzko
Romer Labs
Morgan Wallace
DuPont Nutrition & Health
Testing for Salmonella
MFHPB 20
ISO 6579
Pre-enrichment in
Pre-enrichment in
FSIS (USDA) MLG
4.04
Pre-enrichment in
AOACOMA 200.06,
995.20and 967.26
Pre-enrichment in
FDA BAM
Chapter 5
Pre-enrichment in
Lactose, Nutrient, UP,
BPW
BPW or nutrient broth
Incubation at 37 ºC for
16-20 hours.
Incubation at 35 ºC for
18 to 24 hours
Selective enrichment
in RVS and TBG
broth at 42.5 ºC for
24 h (0.1 mL and
1mL respectively)
Selective enrichment
in RV and MKTTn
Broth. RVS at 37°C
and 42°C and 0.1 mL
and 1 mL respectively
for 24h
BPW
Incubation at 35 ºC for
20 to 24 hours
Selective enrichment
in TTh and mRV,
R10, or RVS broth at
42 ºC for 18-24 h
(0.5 mL and 0.1 mL
respectively)
Lactose or Broth TSB
Incubation at 35 ºC for
24 hours
Selective enrichment in TT,
RV and / or SC broth
depending on method. (1 mL,
0.1 mL and 1 mL respectively)
TT and SC are incubated at
35 ºC and RV is incubated at
42 ºC for 24 hours.
or TS broth. Plus
others
Incubation at 35 ºC for
20 to 24 hours
Selective enrichment
in TT and RV Broth
at 35 ºC and 42 ºC
and 1 mL and 0.1
mL respectively for
24 h. SC broth at
35˚C for Guar Gum
and
S.
Typhi
Streak onto BGS plus
one of DMLIA or XLT
4
Streak on at least 2 of
the 3: Bismuth Sulfite
Agar, BGS, Brilliance
Salmonella agar.
Streak onto XLD and
one other agar (the
second agar is any
agar for the isolation
of salmonella)
Biochemical and
serology tests to
confirm
Biochemicaland
serology tests to
confirm
Biochemicaland
serology tests to
confirm
BismuthSulfite Agar,
HE agar, XLD agar
Biochemical and
serology tests to
confirm
Streak onto HE, XLD,
BismuthSulfite agar.
Biochemicaland
serology tests to
confirm
Slide Courtesy Donna Douey, Canadian Food Inspection Agency