SPDS Lutein and Turmeric ERPs

Reviewer Name:

Yang Zhou

Email:

yangzhou@eurofinsus.com

Organization:

Eurofins

General Information

Method Reviewed:

LUT‐01

Method Title:

QUANTITATIVE MEASURMENT OF BETA‐CRYPTOXANTHIN IN PAPRIKA OLEORESIN,

EXTRACT AND DIETARY SUPPLEMENTS (BEADLETS)

Applicable SMPR

2016.004

Summary of Method:

Based on the sample matrix (paprika oleoresin, extract, or dietary supplement) and

I. Summary

detection instrumentation (HPLC or UV), samples are extracted with corresponding

extraction solvent. Saponification is performed for paprika oleoresin. The extraction

solvent is then quantified by HPLC or UV reading.

1. Does the Applicability of the

Method Support the Applicability

of the SMPR? If not, please explain

No. This method didn't demonstrate its capability to separate and quantify beta‐

cryptoxanthin isomers, lutein and zeaxanthin.

II. Review of the Method Only

what is missing.

2. Does the analytical technique(s)

used in the method meet the

SMPR? If not, please specify how it

differs from what is stated in the

SMPR.

Yes

3. Are the definitions specified in

the SMPR used and applied

appropriatly in the method? If no,

please indicate how the terms are

used.

No. In this method, it specified dietary supplements in beadlets form.

4. Does the method, as written,

contain all appropriate precautions

and warnings related to the

No. Suggested wording: In this method, various organic solvents (hexanes,

chloroform, etc.,) along with several other hazardous chemicals are used and special

care should be taken when handling or disposing of these chemicals. Wear gloves,

method's regaents, components,

instrumentation, or method steps

that may be hazardous? If no,

please suggest wording or

option(s).

safety glasses and use in a fume hood. Follow all laboratory safety precautions. Refer

to Safety Data Sheets (SDS) for any questions relating to any chemicals in use.

1. Are the definitions specified in

No. Reproducibility was replaced by word "ruggedness", which is a completely

III. Review of Information in Support of the Method

the SMPR used and applied

appropriately in the supporting

documentation (manuscripts,

method studies, etc…)? If not,

please explain the differences and

if the method is impacted by the

difference.

different property of a method.