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PDE5-001
Question
Answer
General
AOAC Method Number:
PDE5-001
Method Title:
Screening Method for Phosphodiesterase Type 5 (PDER5)
Inhibitors in Dietary Ingredients and Supplements using
High Resolution Mass Spectrometry
Method Author(s):
Musselman, LaPointe, Crawford and Goguen
Reviewer Name:
Tom Phillips
Summary of Method
The sample is extracted with acetonitrile by rapid mixing
through vortexing. The liquid portion is pipetted onto a
wire mesh sample support and the solvent allowed to
evaporate. The mesh is mounted on the holder and it
moves at a fixed speed between the dart source exit and
the API entrance of the mass spectrometer. The fixed
speed assures there is sufficient time to produce ions for
the mass spectrometer to analyze. Data is collected in
positive and negative ion mode.
Method Scope / Applicability:
Qualitative assay for phosphodiesterase type 5 (PDE5)
inhibitors in dietary ingredients and supplements
including herbs or other botanicals; a dietary substance
for use by man to supplement the diet by increasing total
dietary intake; or a concentrate, metabolite, constituent,
extract, or combination of any of the above dietary
ingredients. {United States Federal Food Drug and
Cosmetic Act §201(ff) [U.S.C. 321 (ff)]}. Dietary
supplements are defined as a product intended for
ingestion that contains a “dietary ingredient” intended to
add further nutritional value to (supplement) the diet.
Dietary supplements may be found in many forms such
as tablets, capsules, softgels, gelcaps, liquids, or
powders. As the supplements are predominantly natural
products the potential for interference is to be minimized
by optimizing the extraction solvent in order to suppress
matrix interference with the assay’s ability to detect
target compounds.