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ANTH-003
Pros/Strengths
1. Chromatography is impressive. The usability of LC with
DAD, MS, or both detection technologies allows
confirmations to occur.
2. Extraction protocol is very simple.
Cons/Weaknesses
1. No work was reported on the method being able to
evaluate the presence of fruits listed in Annex II,
Exclusivity Panel; Selected Non-Vaccinium Fruit.
2. Only one of the seven types of sample matrices was
studied. While I do expect the method to properly
analyze the remaining matrix types, I believe this must be
first demonstrated.
3. Some peaks were not fully characterized but were
"noted as to their type based on MRM information". The
author did mention additional testing on a newer
instrument would likely identify the unidentified peaks.
Supporting Data
General Comments
Chromatograms show acceptable separation of targeted
compounds.
Method Optimization
No study was performed on evaluating if the method can
correctly identify all of the authenticity panel (Annex i)
materials in the presence or absence of fruits listed in the
exclusivity panel (Annex II)
Performance Characteristics
Analytical Range:
Standard calibration curve: 18 to 180 mg/L cyanidin-3-
chloride
LOQ:
N/A for this qualitative method
Accuracy/Recovery
N/A for this qualitative method
Precision (RSDr)
N/A for this qualitative method
Reproducibility (RSDR)
N/A for this qualitative method
System Suitability
No study was performed on evaluating if the method can
correctly identify all of the authenticity panel (Annex i)
materials in the presence or absence of fruits listed in the
exclusivity panel (Annex II)
Safety Review
Is the method safe to use as written?
No specific usage or safety practices listed
Are there any safety concerns with
any component, instrument, reagent
or instruction of the method? If yes,
None were evident outside of handling acids