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ANTH-002
Question
Answer
General
AOAC Method Number:
ANTH-002
Method Title:
Total Monomeric Anthocyanins By HPLC (Artemis
Method)
Method Author(s):
Artemis International / J. Wightman
Reviewer Name:
Martha Jennens
Summary of Method
To provide a documented procedure for the
determination of total anthocyanin by HPLC, with
Iprona/Artemis’s methodology, expressed as cyanidin-3-
glucoside.
Method Scope / Applicability:
This assay can be used to determine total anthocyanin in
the standardized elderberry, chokeberry and bilberry
extracts. This is a reverse-phase HPLC method that can
be used to calculate the total anthocyanin content of an
extract as well as to examine the qualitative “fingerprint”
of the anthocyanins present in the extract.
General Comments About the
Method:
This is a straight-forward dilute and shoot HPLC method.
As written does not cover the identification of all
Vaccinium species, Annex I, outlined in SMPR.
Method Clarity
The method can follow as written, but there are no
QC/acceptability guidelines in the method.
Pros/Strengths
This is a simple, straight-forward method that could be
readily run by most laboratories, using common reagents
and instrumentation.
Cons/Weaknesses
Only one of the two main standards used in this method
(Cy-glu), it should also include(Cy-rut) to be more
complete in the analysis of anthocyanins.
The use of two different wavelengths is not acceptable
when data is being calculated from a different
wavelength than the standard curve. While different
analytes have different optimal maxima, either one
wavelength needs to be selected for use throughout the