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Method Review Form
AOAC INTERNATIONAL Standards Development
Name of Reviewer:
Christine R. Casey
Title of Method:
Identification and Characterization of Indole and Oxindole Alkaloids from Leaves of
Mitragyna speciosa Hroth Using Liquid Chromatography - Accurate QTof Mass Spectrometry
AOAC Candidate Method Number:
KRA-03
Applicable SMPR :
AOAC SMPR 2015.008
I.
SUMMARY OF METHOD
The analytical method was developed to characterize and qualitatively determine the alkaloids from
various M. speciose samples. The method uses two separate extraction procedures: a simple
methanol extraction and an acid-base extraction procedure. Qualitative determination was
performed via a RP C8 column with a water-acetonitrile formic acid mobile phase followed by high
resolution mass spectrometry. The overall objective of the method was to develop a single
qualitative LC-MS/MS ( QTof)method for the separation, characterization, and chemical profiling of
indoles and oxindole alkaloids in Mirtagynine speciosa.
II.
REVIEW OF THE METHOD ONLY:
1.
Does the applicability of the method support the applicability of the SMPR? If no, please explain
what is missing.
Overall, the method does not meet all the requirements to support the AOAC SMPR 2015.008.
Presently, the method does not preform quantitation for 7-hydroxy mitragynine, mitragynine,
system suitability, repeatability, and reproducibility. However, the method does separate 7-
hydroxy mitragynine, mitragynine, and other relevant indoles of Mitragynine speciose.