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Method Review Form
AOAC INTERNATIONAL Standards Development
Name of Reviewer:
Yan-Hong Wang
Title of Method:
LC/MS Method for the Identification of Mitragyna speciose (Kratom) and Quantitation
of Mitragynine Using Linear Ion Trap Mass Spectrometer
AOAC Candidate Method Number:
KRA-04
Applicable SMPR :
I.
SUMMARY OF METHOD
An LC/MS method is developed for quantitative analysis of mitragynine and 7-hydroxymitragynine.
The method has been applied to liquid drinks, liquid tinctures, powders, bulk ground processed
leaves, dried leaves and capsules.
II.
REVIEW OF THE METHOD ONLY:
1.
Does the applicability of the method support the applicability of the SMPR? If no, please explain
what is missing.
The method isn't validated for the parameters precision. The linear range of calibration curve is
not specified.
2.
Does the analytical technique(s) used in the method meet the SMPR? If no, please specify what
how it differs from what is stated in the SMPR.
Yes.