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Method Review Form
AOAC INTERNATIONAL Standards Development
Name of Reviewer:
Catherine (Kate) Rimmer
Title of Method:
LC/MS Method for the Identification of Mitragyna speciose (Kratom) and Quantitation
of Mitragynine Using Linear Ion Trap Mass Spectrometer
AOAC Candidate Method Number:
KRA-04
Applicable SMPR :
SMPR 2015.008
I.
SUMMARY OF METHOD
This method is an LC/MS method for the determination of mitragynine mass fraction and the
identification of Mitragyna speciosa. For the identification several of the alkaloids are separated.
II.
REVIEW OF THE METHOD ONLY:
1.
Does the applicability of the method support the applicability of the SMPR? If no, please explain
what is missing.
Yes
2.
Does the analytical technique(s) used in the method meet the SMPR? If no, please specify what
how it differs from what is stated in the SMPR.
Yes, all methods are acceptable for the SMPR.
3.
Are the definitions specified in the SMPR used and applied appropriately in the method? If no,
please indicate how the terms are used.