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Table 2011.11E. Parameters for UHPLC MS/MS Measurement
Analyte
Q1
a
,
amu
Q3
b
,
amu
Quantifying/Qualifying
Ion
Dwell,
ms
DP
c
EP
d
CE
e
CXP
f
D
3
385.4
259.1
Quantitation Ion
50
55.0
14.5
21.0
17.3
D
3
385.4
107.1
Qualifier Ion
25
55.0
14.5
31.9
5.20
D
3
385.4
159.1
Qualifier Ion
50
55.0
14.5
32.7
9.10
D
2
397.4
125.1
Quantitation Ion
50
43.0
9.5
19.1
6.9
D
2
397.4
107.0
Qualifier Ion
25
43.0
4.5
36.0
19.7
D
2
397.4
271.1
Qualifier Ion
100
43.0
9.0
17.3
18.6
Isotope D
3
388.4
259.1
Internal Standard
50
55.0
14.5
21.0
19.1
Isotope D
2
400.4
125.1
Internal Standard
100
43.0
10
30.0
15.0
a
Q1 = Quadrupole mass filter 1.
b
Q3 = Quadrupole mass filter 3.
c
DP = Declustering potential.
d
EP = Entrance potential.
e
CE = Collision energy.
f
CXP = Collision cell exit potential.
Note: Parameter, except for Mass Spectrometer, Ionization Mode, and Gas Types may be
modified to obtain optimum chromatography and sensitivity. Exact mass ions may vary
slightly from instrument to instrument because of unit resolution of quadrupole mass
spectrometers.
2011.11 VITAMIN D MLT-Sept 2015
FOR ERP USE ONLY
DO NOT DISTRIBUTE