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446
C
rowley
et al
.
:
J
ournal of
AOAC I
nternational
V
ol
. 97, N
o
. 2, 2014
purified and inactivated
L. monocytogenes
receptors +
preservative + protein stabilizer.
(
e
)
Positive control solution.—
1×3 mL. Contains purified and
inactivated
L. monocytogenes
antigen + preservative + protein
stabilizer.
(
f
)
Negative control solution.—
1×6 mL. Contains Tris-
buffered saline (TBS; 150mmol/l) – Tween pH 7.6 + preservative.
(
g
)
Master lot entry
(
MLE) card.—
One card providing
specifications for the factory master data required to calibrate the
test: To read the MLE data, please refer to the Operator’s Manual.
(
h
)
Package insert
.
Table 2013.11B. Summary of results for the detection of
Listeria monocytogenes
in queso fresco (125 g)
Method
a
VIDAS LMX w/OXA
VIDAS LMX w/ALOA
Inoculation level
Uninoculated
Low
High Uninoculated
Low
High
Candidate presumptive positive/total No. samples analyzed 0/144
70/144
144/144
0/144
70/144
144/144
Candidate presumptive POD (CP)
0.00
0.49
1.00
0.00
0.49
1.00
(0.00, 0.03)
(0.40, 0.57) (0.97, 1.00)
(0.00, 0.03) (0.40, 0.57) (0.97, 1.00)
s
r
b
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.16)
(0.46, 0.52) (0.00, 0.16)
(0.00, 0.16) (0.46, 0.52) (0.00, 0.16)
s
L
c
0.00
0.00
0.00
0.00
0.00
0.00
(0.00, 0.16)
(0.00, 0.12) (0.00, 0.16)
(0.00, 0.16) (0.00, 0.12) (0.00, 0.16)
s
R
d
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.22)
(0.46, 0.52) (0.00, 0.22)
(0.00, 0.22) (0.46, 0.52) (0.00, 0.22)
P
value
e
1.0000
0.9730
1.0000
1.0000
0.9730
1.0000
Candidate confirmed positive/total No. samples analyzed
0/144
70/144
144/144
0/144
70/144
144/144
Candidate confirmed POD (CC)
0.00
0.49
1.00
0.00
0.49
1.00
(0.00, 0.03)
(0.40, 0.57) (0.97, 1.00)
(0.00, 0.03) (0.40, 0.57) (0.97, 1.00)
s
r
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.16)
(0.46, 0.52) (0.00, 0.16)
(0.00, 0.16) (0.46, 0.52) (0.00, 0.16)
s
L
0.00
0.00
0.00
0.00
0.00
0.00
(0.00, 0.16)
(0.00, 0.12) (0.00, 0.16)
(0.00, 0.16) (0.00, 0.12) (0.00, 0.16)
s
R
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.22)
(0.46, 0.52) (0.00, 0.22)
(0.00, 0.22) (0.46, 0.52) (0.00, 0.22)
P
value
1.0000
0.9730
1.0000
1.0000
0.9730
1.0000
Positive reference samples/total No. samples analyzed
0/144
69/144
144/144
0/144
69/144
144/144
Reference POD
0.00
0.48
1.00
0.00
0.48
1.00
(0.00, 0.03)
(0.39, 0.56) (0.97, 1.00)
(0.00, 0.03) (0.39, 0.56) (0.97, 1.00)
s
r
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.16)
(0.46, 0.52) (0.00, 0.16)
(0.00, 0.16) (0.46, 0.52) (0.00, 0.16)
s
L
0.00
0.00
0.00
0.00
0.00
0.00
(0.00, 0.16)
(0.00, 0.12) (0.00, 0.16)
(0.00, 0.16) (0.00, 0.12) (0.00, 0.16)
s
R
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.22)
(0.46, 0.52) (0.00, 0.22)
(0.00, 0.22) (0.46, 0.52) (0.00, 0.22)
P
value
1.0000
0.9672
1.0000
1.0000
0.9672
1.0000
dLPOD (C vs R)
0.00
0.01
0.00
0.00
0.01
0.00
(–0.03, 0.03) (–0.10, 0.13) (–0.03, 0.03)
(–0.03, 0.03) (–0.10, 0.13) (–0.03, 0.03)
dLPOD (CP vs CC)
0.00
0.00
0.00
0.00
0.00
0.00
(–0.03, 0.03) (–0.12, 0.12) (–0.03, 0.03) (–0.03, 0.03) (–0.12, 0.12) (–0.03, 0.03)
a
Results include 95% confidence intervals.
b
Repeatability standard deviation.
c
Among-laboratory standard deviation.
d
Reproducibility standard deviation.
e
P
value = Homogeneity test of laboratory PODs.
Candidates for 2016 Method of the Year
347