174-175 / 350
February 20, 2013
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Matrix
Screen for
Indigenous
Salmonella
Estimate
total aerobic
counts
0.1ml sample + 10ml mRV
0.5ml sample + 10ml TT (Hanja)
22-24h at 42 ± 0.5°C
• Streak to XLT4 (or DMLIA) & BGS
Incubate at 35±2°C
Examine at 18-24h & 42-48h
Transfer typical colonies to TSI/
LIA slants
24±2h at 35±2°C
Ground chicken, pork, beef:
25g + 225mL*
Liquid egg:
100g + 900 mL*
Cooked chicken nuggets
: 325g + 2925mL*
Homogenize 2 min
*BPW
Biochemical/serological
confirmations on all enrichments
according to USDA/FSIS
MPN
3M
USDA
Raw ground chicken, pork, beef
: 25g + 225mL*
Liquid egg
: 100g + 900 mL*
Cooked chicken nuggets
: 325g + 2925mL*
Homogenize 2 min
18-24h at 41.5±1°C
*3M Salmonella Enrichment + Supplement
(prewarmed to 41.5C)
Ground chicken
20-24h h at 35±2°C
Liquid eggs
18-24h h at 35±2°C
Run the 3M Petrifilm
Salmonella Express
System
Transfer typical colonies to
TSI/LIA slants
24±2h at 35°C
Biochemical/serological
confirmation on all
enrichments according to
USDA/FSIS
Pick typical colony and
transfer to TSI/LIA slants
24±2h at 35°C
Biochemical/serological
confirmation on all
enrichments according to
USDA/FSIS
0.1ml sample + 10ml mRV
0.5ml sample + 10ml TT (Hanja)
22-24h at 42 ± 0.5°C
• Streak to XLT4 (or DMLIA) & BGS
Incubate at 35±2°C
Examine at 18-24h & 42-48h
Chicken, pork, beef
Transfer 0.1 mL BPW
to 9.9 mL RV10
8 h at 41.5±1°C
Egg
Figure 1. 3M Petrifilm
Salmonella
vs. USDA – raw ground chicken, raw ground beef, raw ground pork, chicken nuggets, and
1
pasteurized liquid whole eggs.
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