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February 20, 2013
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Figure 3. 3M™ Petrifilm™ Salmonella Express vs. FDA-BAM – stainless steel
1
Surface
1 sponge + 225ml
Lactose broth,
Mix well and let stand 60±5 min at room temp
Mix and adjust pH to 6.8±0.2 if necessary
24±2h h at 35°C
0.1ml sample
+ 10ml RV, 24±2h at
42±0.2°C
Transfer typical colonies to
TSI/LIA slants
24±2h at 35°C
1.0ml sample
+ 10ml TT, 24±2h at
35±2°C
BAM
Biochemical/serological
confirmation on all
enrichments according to
FDA-BAM
• Streak to XLD & HE - 24±2h at 35°C
• Streak to BS - incubate at 35°C for 24±2h,
read and pick if applicable.
Re-incubate another 24 h
Sample surface with swab or sponge and
store in DE broth 2hr at room temp
Run the 3M Petrifilm Salmonella
Express System
3M
1 sponge + 225ml
3M BPW ISO
(prewarmed to 42C)
Homogenize 2 min
18-24h h at 42±1°C
Transfer typical colonies to
TSI/LIA slants
24±2h at 35°C
Biochemical/serological
confirmation on all
enrichments according to
FDA-BAM
• Streak to XLD & HE - 24±2h at 35°C
• Streak to BS - incubate at 35°C for
24±2h, read and pick if applicable.
Re-incubate another 24 h
Pick typical colony and
transfer to TSI/LIA slants
24±2h at 35°C
Biochemical/serological
confirmation on all
enrichments according to
FDA-BAM
0.1ml sample
+ 10ml RV, 24±2h at
42±0.2°C
1.0ml sample
+ 10ml TT, 24±2h at
35±2°C
2
3
4