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Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Table 11. 3M Petrifilm SALX Method Comparison Chi-Square Results for Raw Ground Chicken vs USDA/FSIS MLG (Internal Data)

1

SALX

Confirmation

Disk Positive Reference

Control

N/A

< 0.075

5

0

0

0

0

-

0.2-2 CFU/

S. enterica

ser.

Heidelberg

1.15

Test Portion NCTC 5717

(0.70-2.07)

Control

N/A

< 0.075

5

0

0

0

0

-

0.2-2 CFU/

S. enterica

ser.

Heidelberg

1.15

Test Portion NCTC 5717

(0.70-2.07)

Control

N/A

< 0.075

5

0

0

0

0

-

0.2-2 CFU/

S. enterica

ser.

Heidelberg

1.15

Test Portion NCTC 5717

(0.70-2.07)

Control

N/A

< 0.075

5

0

0

0

0

-

0.2-2 CFU/

S. enterica

ser.

Heidelberg

1.15

Test Portion NCTC 5717

(0.70-2.07)

USDA/FSIS

MLG

Presumptive

positive test

portions on

3M Petrifilm

SLAX Plates

Confirmed

18 Hr Primary Enrichment – 8 Hr RV-R10 2

o

Enrichment

X

2

Alternative

Confirmation

X

2

Traditional

Confirmation

Inoculation

Level

Inoculating

Organism

APC

CFU/g MPN/25g*

Total

Samples

3M Petrifilm SALX Method

7.1 x 10

3

-

20

15

15

14

14

0.12

0

18 Hr Primary Enrichment – 24 Hr RV-R10 2

o

Enrichment

7.1 x 10

3

-

20

15

15

14

14

0.12

0

24 Hr Primary Enrichment – 8 Hr RV-R10 2

o

Enrichment

7.1 x 10

3

-

20

15

15

14

14

0.12

0

24 Hr Primary Enrichment – 8 Hr RV-R10 2

o

Enrichment

7.1 x 10

3

-

20

15

15

14

14

0.12

0

2

*Includes 95% confidence interval

3