305 / 350
One non-target strain,
E. coli
ATCC 25922,was tested at a high level inoculum level (target 150 cfu/mL).
1
For the yeast
Candida troplicalis
, five replicates were inoculated to Petrifilm RYM Plates achieve the
2
target level of approximately 50-100 CFU/mL for high level and 10-20 CFU/ml for the low level. For
E.
3
coli
, five replicates were inoculated to achieve target levels of 100-150 CFU/mL for the high level
.
The
4
plates were incubated at 25 ± 1
º
C for 48 hours and then the colonies were enumerated.
5
6
Table 4.
Summary of Combined Shelf Life and Lot-to-Lot Consistency for
Geotrichum candidum
at the
7
Low Inoculation Level.
8
DRBC Raw
Count
Log
10
Petrifilm
2014-01 KA
Raw Count
Log
10
Petrifilm 2014-
01 KB Raw
Count
Log
10
Petrifilm 2014-
01 KC Raw
Count
Log
10
14
1.146
17
1.230
21
1.322
13
1.114
24
1.380
14
1.146
11
1.041
21
1.322
15
1.176
17
1.230
21
1.322
20
1.301
18
1.255
15
1.176
13
1.114
16
1.204
18
1.255
15
1.176
14
1.146
15
1.176
1.243
1.192
1.189
1.223
t-test 0.422
0.608
0.631
9
10
Table 5.
Summary of Combined Shelf Life and Lot-to-Lot Consistency for
Geotrichum candidum
at the
11
High Inoculation Level.
12
DRBC Raw
Count
Log
10
Petrifilm 2014-
01 KA Raw
Count
Log
10
Petrifilm 2014-
01 KB Raw
Count
Log
10
Petrifilm
2014-01 KC
Raw Count
Log
10
43
1.633
42
1.623
47
1.672
58
1.763
45
1.653
55
1.740
48
1.681
54
1.732
54
1.732
46
1.663
51
1.708
45
1.653
33
1.519
48
1.681
50
1.699
50
1.699
40
1.602
56
1.748
57
1.756
46
1.663
1.628
1.691
1.703
1.702
t-test 0.232
0.128
0.164
13
14
15
16
17
18
19
20
21
22
Table 6.
Summary of Combined Shelf Life and Lot-to-Lot Consistency for
Candida tropicalis
at the Low
23
Inoculation Level.
24
9
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014