303 / 350
24°C 44 and 62 hours
C. tropicalis -
LOW
Inoculation
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev
0.1% Peptone Water
16 16 23 16 15 17.200 3.271 17 16 24 17 16 18.000 3.391
0.1% Peptone water (45°C)
17 17 13 21 18 17.200 2.864 18 18 13 21 19 17.800 2.950
Butterfields Buffer
28 21 18 18 20 21.000 4.123 28 21 19 18 20 21.200 3.962
Letheen Broth
20 25 31 16 17 21.800 6.221 21 26 31 17 18 22.600 5.857
Saline
7
12
6
18 11 10.800 4.764
8
12
6
18 12 11.200 4.604
Peptone Salt Diluent
19
8
15 17 12 14.200 4.324 19
9
15 17 14 14.800 3.768
Water
11 15
7
18 18 13.800 4.764 11 15
8
19 19 14.400 4.879
BPW
12 15 15 17 13 14.400 1.949 13 15 15 18 13 14.800 2.049
29°C 44 and 62 hours
C. tropicalis
- LOW
Inoculation
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev
0.1% Peptone Water
18 26 29 16 19 21.600 5.595 18 26 29 17 19 21.800 5.357
0.1% Peptone water (45°C)
23 18 22 14 18 19.000 3.606 23 18 22 14 19 19.200 3.564
Butterfields Buffer
16 16 11 13 21 15.400 3.782 16 16 11 13 21 15.400 3.782
Letheen Broth
18 17 18 20 20 18.600 1.342 20 17 18 20 22 19.400 1.949
Saline
7
12 14 11 16 12.000 3.391
7
12 14 10 15 11.600 3.209
Peptone Salt Diluent
24 21 16 14 11 17.200 5.263 24 21 16 14 11 17.200 5.263
Water
11 13 16 27 14 16.200 6.301 11 13 16 27
16.750 7.136
BPW
9
17 22 15 21 16.800 5.215
9
16
2
15 21 12.600 7.301
24°C 44 and 62 hours
C. tropicalis
- HIGH
Inoculation
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev
0.1% Peptone Water
59 65 65 62 65 63.200 2.683 60 66 65 63 66 64.000 2.550
0.1% Peptone water (45°C)
49 56 59 57 72 58.600 8.385 50 59 62 60 74 61.000 8.602
Butterfields Buffer
52 45 58 49 63 53.400 7.162 54 45 62 50 63 54.800 7.727
Letheen Broth
47 64 54 48 50 52.600 6.914 47 64 56 50 50 53.400 6.768
Saline
44 39 41 50 38 42.400 4.827 46 39 42 50 38 43.000 5.000
Peptone Salt Diluent
56 58 41 36 35 45.200 11.032 56 61 44 36 35 46.400 11.718
Water
29 38 36 51 30 36.800 8.815 32 42 46 52 31 40.600 9.044
BPW
40 36 39 42 44 40.200 3.033 41 37 39 42 46 41.000 3.391
29°C 44 and 62 hours
C. tropicalis -
HIGH
Inoculation
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Average Sdev
0.1% Peptone Water
59 60 80 64 69 66.400 8.562 60 60 82 65 70 67.400 9.154
0.1% Peptone water (45°C)
58 72 65 63 54 62.400 6.877 57 72 66 64 57 63.200 6.380
Butterfields Buffer
50 53 59 44 63 53.800 7.463 50 52 59 44 63 53.600 7.503
Letheen Broth
60 49 52 55 65 56.200 6.380 61 50 51 55 65 56.400 6.465
Saline
36 40 52 42 37 41.400 6.387 35 41 53 42 36 41.400 7.162
Peptone Salt Diluent
43 50 44 38 45 44.000 4.301 43 49 45 39 45 44.200 3.633
Water
45 37 35 39 32 37.600 4.879 45 37 35 39 32 37.600 4.879
BPW
53 52 42 51 60 51.600 6.427 53 52 42 50 59 51.200 6.140
44 hours
62 hours
44 hours
62 hours
44 hours
62 hours
44 hours
62 hours
1
2
3
4
5
6
7
8
9
10
Table 3.
Summary of Ruggedness Test Results for
Geotrichum candidum
– Inoculation Level, Incubation
11
Time, Incubation Temperature, and Dilution Fluid.
12
7
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014