300 / 350
Additional Supplies and Reagents
1
1.
Pipettor capable of pipetting 1,000µl or sterile serological pipette
2
2.
Sterile pipette tips
3
3.
Sterile diluent
4
4.
3M™ Petrifilm™ Flat Spreader (6425)
5
6
Apparatus
7
Incubator capable of maintaining 25-28 ± 1°C.
8
9
Safety Precautions
10
3M Petrifilm RYM Plates do not differentiate any one yeast or mold strain from another.
11
3M has not documented 3M Petrifilm RYM Plates for use in industries other than food and beverage.
12
For example, 3M has not documented 3M Petrifilm RYM Plates for testing water, pharmaceuticals or
13
cosmetics. 3M Petrifilm RYM Plates have not been tested with all possible food products, food
14
processes, testing protocols or with all possible strains of yeast and mold.
15
Do not use 3M Petrifilm RYM Plates in the diagnosis of conditions in humans or animals.
16
The user must train its personnel in proper testing techniques. For example, Good Laboratory
17
Practices
2
, ISO 7218
3
, or ISO 17025
4
. After use, 3M Petrifilm™ RYM Plates may contain microorganisms
18
that may be a potential biohazard. Follow current industry standards for disposal.
19
20
21
General Preparation
22
Use proper aseptic technique. Use proper precautions for biosafety level 2 microorganisms.
23
Store unopened packages at ≤8°C (≤46°F). Use before expiration date on package. In areas of high
24
humidity where condensate may be an issue, it is best to allow packages to reach room temperature
25
before opening.
26
27
Sample Preparation
28
When selecting a test method, it is important to recognize that external factors such as sampling
29
methods, testing protocols, sample preparation, handling, and laboratory technique may influence
30
results.
31
It is the user’s responsibility in selecting any test method or product to evaluate a sufficient number of
32
samples with the appropriate matrices and microbial challenges to satisfy the user that the chosen test
33
method meets the user’s criteria.
34
4
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014