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K
line
et al
.:
J
ournal of
AOAC I
nternational
V
ol
.
100, N
o
.
3, 2017
Figure 3. Aloe-emodin region overlay HPLC chromatograms of (a) 20 ppb standard mixture, (b) solvent blank, (c) placebo of the powder
product, (d) placebo of the liquid concentrate product, (e) 20 ppb standard mixture-spiked placebo of the powder product, and (f) 20 ppb
standard-spiked placebo of the liquid concentrate product.
Table 1. Precision test for a typical lot of aloe vera 5× raw
material
Lot No.
Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb
1
ND
a
ND
ND
2
ND
ND
ND
3
ND
ND
ND
4
ND
ND
ND
5
ND
ND
ND
6
ND
ND
ND
Avg.
NA
b
NA
NA
RSD, %
NA
NA
NA
a
ND = None detected.
b
NA = not available.
Table 2. Precision test for a typical lot of aloe vera 200×
raw material
Lot No.
Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb
1
345.9
325.5
22.9
2
355.1
351.5
26.6
3
340.6
327.9
23.7
4
349.1
330.1
22.1
5
348.8
330.1
24.1
6
341.4
333.9
21.6
Avg.
346.8
333.2
23.5
RSD, % 1.6
2.8
7.5
Table 3. Precision test for a typical lot of aloe concentrate
product
Lot No.
Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb
1
ND
a
ND
ND
2
ND
ND
ND
3
ND
ND
ND
4
ND
ND
ND
5
ND
ND
ND
6
ND
ND
ND
Avg.
NA
b
NA
NA
RSD, %
NA
NA
NA
a
ND = None detected.
b
NA = not available.
Table 4. Precision test for a typical lot of aloe powder
Lot No.
Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb
1
197.0
198.8
<20
2
198.7
202.7
<20
3
206.2
205.4
<20
4
201.4
206.6
<20
5
202.8
208.7
<20
6
201.3
207.7
<20
Avg.
201.2
205.0
NA
a
RSD, %
1.6
1.8
NA
a
NA = not available.
8