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I30
I-r.;po
I't'AI-.:JOIjRNÂI.
OITAOACI
I¡itERN^.ftoNr\t-
VL
96.
No.
1.2013
Table
13.
Rice
flour
accuracy and
precision
from
independent Iaboratory
F¡nal result.
ppm
Recovery.
%
Replic¿te
Target
Q
l.abs
Neogen
concn,
(OL)
sample
lD
Dilution
ppm
lD
No.
No.
faclor
0.0
15
11n
12ã
13n
14n
15n
20.0
16
tt
18
40
20
16n
17n
18n
19n
20n
40.0
21
23
1.0
0.0
0.0
1
.0
0.0
0.0
'L0
0.0
0.2
1.0
1.3
0.0
'1
,0
0,3
0.2
'1
.0
0.1
0.1
1.0
0.6
0.3
'1.0
0.2
0.2
1,0
0.0
0.0
1.0
0.4
0.3
1.0
6.6
7.3
1.0
e.4
10.0
1
.0
6.4
6.4
1.0
7.7
8.1
1.0
8.0
6.9
1.0
4,8
4.6
1.0
5,1
4.1
1
.0
4.1
4.8
1.0
5.4
4.6
1.0
4.3
4,5
1_0
11.8
11.1
1.0
11.3
11.'l
'1.0
11.1
10.6
1.0
11.3
11.0
'1.0
12.1
12.9
'1.0
7.7
7.2
1.0
8.8
8.8
'1.0
7.3
8.9
1.0
7.7
7.9
1_0
8.5
8.9
1.0 't6.?
1
5.6
1.0
?1
,2
20.4
1
.0
'1
9.0
19.8
1.0
19.5
17.3
1.0
1
9.5
19.9
1.0
23,5
18.8
1.0
21
.5
23.3
'1.0
22.5
22.8
L0
24.0
22.1
1.0
23.2
21.6
1.0
3ô.8
40.8
1.0
40.4
37.1
1.0
37.4
38.1
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
132
146
1
88
200
128
128
154
162
160
138
96
92
'to?
82
82
90
108
92
86
90
118
111
113
111
111
1
06
'r13
110
121
129
77
72
88
88
73
89
77
79
85
89
81
78
106
102
95
99
97.5
86.5
97.5
99.5
119.5
94
107.8
1
16.5
112.5
114
120
110.5
116
I
08
92
102
101
92.8
93.5
95
2
1
2
3
4
1n
2n
3n
4n
5n
6
7
I
I
10
6n
7n
8n
9n
'l0n
11
12
13
14
2518
457
794
4450
249
41
63
4976
436'1
602
937
2948
1
038
147
5
4051
4983
4460
3117
1
490
1
539
1426
3270
3830
2868
3739
2709
2895
2846
¿ö40
3'135
3683
1
693
2340
3830
1
019
2805
4591
1777
431
5
2447
3
851
NA
NA
NA
NA
NA
NA
NA
NÅ
NA
NA
5.0
10.0
Table
13.
(continuedl
Final
result.
ppm
Recovêry,
%
Replicate
Target
Q
Labs
Neogen
concn,
(OL)
sample
lD
D¡lution
ppm
lD
No.
No.
factor
84
92.8
93
96.3
92
96
97.3
1
02.8
103
107
.8
96.3
94.8
"
NA
= Not applicable.
ol-three spiked
samples
ofgluten-fiee
corn
(0,5.
and
l0
ppnr)
tested
over
thlee conditions
each.
over
four dil'ferent
paranìrlels
each. ancl
ovcr
2
ditvs. J'hc threc
condilions
rcpreserlt
a
lo\\.
nìediurn. and
lrigh
valL¡e o1-each
pararneter. Parameters include
teurperature.
shaking
lirne.
reagent
volurne.
and
incubation
tirne. Gluten-fiee
cont
rvas
spikcd using
refèrence
material
described
in
ll.e.ference
,\'lalerial
Used
in Sluntlard
Prep(u'dt¡on
and Spiking
sectiorl
of
this
docurnent.
All
santples u'ere
blind-
coded.
(a)
TÞnryteruturz.-All
parârìreters
of
the
kit
'nere uscd
as
indicated
in
Sant¡tle
Preparaliott
ctnd
Lttraction,
Procedut'¿tl
l\'otes,
Precaution,ç,
Anall'sis, and InÍerpretotion
01'
Results
sections
of
this
document.
rvith
the
exception
of
assa¡,
ternperature.
shich
u,as
intenlionalll,
altered
to
include
18. 24.
and
30oC.
'lhese lernperatule diffèrences
appear
to
have
little
elÈct
on
results
uhen
cor-npared
to
the
optimal
tenrperature
ol
24"C
(Table
5).
(b)
ReagenÍ
yolunte.-All
pararneters
oi
the
kit
u,ere
used
as
indicated
in Santple
Preparalion and Et¡tractiott,
Proce¿lural
A'otes,
Precautions,
Anuhtsis, and Interpretatron
of
Results
sections
olthis
document.
rvith
the
exception of-reagent volume
lor
all
steps.
rvhich
rvas
intentionall¡,
allered
to include 95.
100.
and
105
¡rI-. Data indicale
fhese
dif'feretrces
in
volurne
havc
little
elïect on
l'e
sults
shen
compared
to the
optimunl
voluntc
of
100
¡l-
(Table
6).
(e)
Shaking
linte.-All
pararneters
oi
the
kit
tere
used
as
irrdicafed
h
,Somple
Prcpuralion ond
Ltlraction,
Procetlurul
l¡'otes, Precautions, ¡lnal.\,sis,
and lnterpretation oJ'
Rentlts
sections
olthis
docurrent.
rvith
the
cxception of'shaking tinre
lbr
both courpetitiorì and
substrate steps.
rrhich
rvas
intentionallr,
altered
to include
no
shaking.
l0
s,
and 20
s.
l'hese
diflerences
appear to have
Iittle elìèct
on rr:sults (Table
7).
(d)
lncubation
tinte.-AII
pat'alìleters
of
the
kit
u'ere
used
as
indicated
in Satrtple Prepat'atiort
and
Ertractiott,
P¡r¡ce¿lurtl
I'otes.
Precaulicttts.
Anal.y.sis.
and lnlerpretotion
o.f'
Il.esults
sectious
ol'this
document- q'ill-r
tlre
exception
of
incubation
lirne
lor all
steps.
u,hich
rvas
intentionally
altered
to
include
8
min.
l2
min,
and
acornbination
of 8.
10,
and
l2
min.
T'hcse
diflèrences
appe¿ìr 1o
have
little
ellèct on
results.
\\'hile
Iiltlc
el'Íèct u,as obscrvcd- an
optirnunr
tirning
<ll'l0
nrin/step
is
still
recomnrended
(lhble
8).
24
25
21n
22o
23n
24n
25n
2459
1105
3165
1897
178
1
600
2A
1.0
1.0
2.O
'1.0
2.0
2.O
33.6
37
.1
49.4
55.0
37.2
38.7
36.8
38.4
38.9
41.1
41.2
23.1
38.5
37.9