65 / 112
Table
10.
Gluten-free rice cereal accuracy and
precision
from
independent laboratory
Final result.
ppm
Recovery,
%
Replicate
Target
concn
ppm
Neogen
sample
Dilut¡on
lD
No.
lD
No-
factor
0.0
1
1887
2
3172
3
4047
4
2994
5
tót
iii
iúL
2n
2809
3n
3173
4n
3277
5n
4879
6
612
7
2594
I
1024
I
238ô
1
0
4500
6n
1134
7n
1415
8n
2022
9n
2803
10n
4694
11
3026
12
1975
13
1482
14
2202
15
158'1
'11
n
879
12n
1398
13n
2882
14n
401
I
15n
4529
.r
6
1472
17
1082
18
1073
19
1512
20
4656
16n
31
3
17n
696
1
Bn
1514
19n
17?5
20n
2779
21
375
22
1475
23
265
'1.0 0.8
0,0
1.0
0.3
0.2
.r.0
2.0
0.1
1.0
0.8
0.5
1
.0
0.3
0.2
1.C
C.3
'¡.!,4
1
.0
0.0
0.
16
1_0 0.0
0.22
1.0
0.0
0,0
1.0
0.8
0.0
1.0
6.9
6.8
1.0
6.6
ô.6
'1.0 5,6
5.8
1.0
6.9
7.5
1.0
5.7
6.8
1,0
5.2
4.7
1.0
5.0
4.7
'1.0 4.9
4.6
1.0
5.0
5_4
1.0
4.9
5.2
1.0
16.1
15.9
1.0
'10.0
9.2
1.0
8.5
8_1
1.0
9.9
8.6
1
.0
8.3
8,4
1.0
8.7
7
.5
'1
.0
9.0
8.7
1.0
7.4
9.8
'1.0 8.2
9.2
1
.0
7
.6
9.'l
'f
.0
21.4
20.3
1.0
?4.8
24.4
1.0
29.O
30.2
1
_0
14.9
14.5
1.0
12.7
12.2
1.0
23.0
18.4
1.0
?2.0
21
.6
1.0
22.9
20.7
1.0
21.2
14.02
1.0
21.7
2?.1
2.0
26.2
27.8
1.0
32.3
32.6
2.0
32.6
33.0
NA"
NA
NA
NA
NA
NA
NA
NA
NA
NA
iìÅ
üA
NA
NA
NA
NA
NA
NA
NA
NA
138
136
1
30
132
112
116
138
150
114
1
36
104
94
100
94
98
92
100
108
98
104
161
159
100
92
85
81
83
84
87
75
90
87
74
98
82
92
76
91
107
1
01.5
124
121.5
145
'f5l
74.5
72.5
63.5
61
115
92
110
108
'11
5
104
106
70.1
109
110.5
65.5
69.5
80.8
81.5
81
5
825
10.0
40.0
[-r.'po
t,'t'^t-.:
Jot-rtìNAL.
ot.
AO¡\(]
INt-trRNAt-toN^l-
VOt..
!X).
i*vo.
l.
20l
l
127
Table
10.
(continuedl
Final result.
ppm
Recovery,
%
Replicate
Target
concn,
ppm
Neogen
sample
D¡lution
lD
No.
lD
No.
factor
2.O
33.8
31 .4
2.O
29.2
28.8
1.0
46_7
52.6
1.0
40.1
50.8
¡.u
!l_Y
+J.o
1.0
47.5
48.9
1.0
46.0
49.0
84.5
7A
'f
16.8
131.5
100.2
127
129.7
1il9_5
11
8.8
122.3
115
'122.5
'
NA
= Not appl¡cable.
telìrperatr¡re
(
l8-30'C-
64-86'F).
Discald
the
red-tnarked
transler
uells.
(f¡
tìmpty
tlìe
cor)terlts
of
the
riells
into
a
sink.
With
a
u'ash
bottlc.
fìll
cach
antibod\'\\'cll
\rith
the ç'ash
bulììr
solution
anrl
durnp
out.
Re¡reat
the
\\'ashir-tg
fìve
tinles. lhen
turn
the
q,ells
upside
do\ur
and tap
oul
on
¿ì
paper
lou.elr¡n1il
âll
u.ashing
solìition
is removed.
(g)
Pour the
needed
volurne
ol
coniugate
tionl
the
blue-
labeled
bolfle into
a
clcan reagent
boat.
(h)
LJsing
the
l2-chanuel pipcttor and ne\\, tips,
transfèr'
100
pL conjugate into
all
the
tells
and
rnix
f'or 20
s
by,
sliding
the
uell
holder
back and f'orth on
a
flat
sr¡rlàce.
(i)
Inculrate
f-or
l0
urin
at arntrient
tel.nperature.
f-i)
Wash
all
rvells
rrith
the
rvash
buffer solulion
as
described
previously.
(k)
Pour
tlte
needed
volulne
ol
substrate
solutioll lrorn
the
gleen-labeled bottle into
a
clean reagent
boat.
(l)
Place
neu
tips on
the l2-channel
pipettor,
transler
|
00 ¡rL
substrate
into
each
rre
ll.
and
rnix
f'oL
20
s.
(m)
lncubate
lor l0
rnin
at ambient ternperature.
(n)
Pour the
needed
volutre of
Red Stop solution
ftonl
the
red-labeled
botlle
inlo
a
clean reagent
boat.
(o)
Place
neri
tips on
the l2-channel
pipertor.
franslcr
100
¡tl-
Red Stop
inlo
each
rvell.
and
mix lor
20
s.
(p)
S:ipe
the
bottonl
ol
the
microrvells
and read
in
a
nlicloucll
rcader
rvilh
a
ó50
nnl
fìlter.
(q)
Interpret
tlìe
test's
results using Neogen's
Stat
Fax
tnicrouell
reader.
or
an
equivalenl
sTri¡t
reader.
If
using
a
strip
reader.
calculate
lhe
results using Neogen's Veratox solÌware
for
Windous.
I
nte
rp
retati
on
ol
Resu/fs
Iìcsults
betrl'een
2.5
and
40 ppnr
are
qLrantitative. Resuhs
greâfer
than .f0 ppnr should
be
diluted
lìlr
proper
quanlitation.
'f
he
standards
are
ple¡rale
d
lì'onl
u'hcat
gliadin and
are
calculatcd
as
ppm gliadin,
Approxinratcl¡,50%
olthe
gluten
is
available
as
gliaclin.
l'herelore.
to calculate
the
qluten value
ol
the samples.
multiplv
the
ppnì
gliadin
results b¡'
2
ilclesired.
For
the
pur-poses
of'this
validation
stud1.
all
results u
ill
be
repoltccl
as
gliadin
even
if
they are
belo\\'the
los'est
caliblatol'.
?4
3371
?5
3757
?1n
1644
22n
2335
24n
3274
25n
4A47