64 / 112
126
l-r'po
[]l'
At,.:
.lOtJIìNAL
ot.
AO¡\C
INI-l'lrxrrllOul
Table
6.
Robustness of volume
Sanrples,
pÞm
Volume,
pL
10
Run No.
1
95
100
105
0.00
0.00
0.00
5.30
4.90
430
L60
10.60
8.70
Run No.
2
100
105
0.00
0.00
0.00
5.20
5.70
440
9.90
9.60
8,80
Table
7.
Robustness of
shaking
effects
Samples,
pprn
Shaking,
s
Run No.
1
No shake
(0)
10
20
0.40
0.00
0.00
4.80
4.10
4.60
L70
9.20
9.00
Run No.
2
No
shake
(0)
10
20
0.40
0.00
0.00
4.70
4.50
460
9.50
8.50
8.10
Tabfe
8.
Robustness of assay time
V¡r-.
96.
No
l.20l3
tightly
closcd. and
kce¡r
arva,r'
lronr
hcat,
sparks.
open
flatìrù.
and those
snroking.
It
is
toxic if'sriallorvecl
or if-r'apor
is
inhalcd.
Avoid
contacl
q,ith
skin.
(b)
Coniponerlls
of-
Verato\ R5 Gliadin tcst
kit.
such
as
controls
and
cxlrac(ion additivc. rna¡'conlain
onc
or
tìlorc
ofthe
lollou ins
potentiall¡' allcrgic materials: gluten.
casein. alnlond
protein.
and
so)
protein.
If'allergic
to
anv
of'these
conrpounds.
be
cautious s,hen using this prodtrct.
(c)
Store
test
kit
bet\\,cen
2-8"C (35-4(r'F)
u,lien not in
usc.
Do not
lìeeze test
kits.
and
avoid prolonged
slorage
¿ìt
arnbienr
tcrìl
peralures.
(d)
Iìrin,a
kits
1o
anrbient tenrperaturc
(18-30'C.
64-86"1'')
priol
to
usc.
(e)
Do
not
usc
kit
cornponents be¡,ond
erpiration
date.
(f)
Do
¡rot
rnir
reagents
fiont
one
kit
lot
rvith
reagents
liom
a
tiific¡,;¡¡i
ioi.
(g)
Use clean
pipet tips
and slassware
fòr
cach sample
to
avoid
cross-conlarnination.'l-horou_ehl¡,
rlash
all
glassrvale
bel$'een
samplcs.
Analysis
(a)
llenrove onc rcd-nlarked mi-ring
vcll
lbr
each
saurplc
ro
bc
tested,
plus fìr,e
f-or
controls. and place
in
the
uell
holder
(strips consist
of
I
2 ri
ells
and are breakable).
(b)
Rcmove
an
equal number anlibodr,-coated rvells. Return
antibody
rvells
lhat
rvill
not
be used
immediatel¡'to
the
f'oil
pack
rvilh
desiccant.
A
nrinìmurrr
of
selen
u'ells
are necessar;'
tbr
a
single sample
(stlips
consist
ol'
l2
rvells and are breakable).
(c)
Using
a
nerr
pipet
tip
f'or each.
transfer
150
pL
of controls
and
sanrple
extracts
to
the red-nìarked translèr rvells
(transfèr
viells
are
used
to
stage the
assal'to eliminate end-to-cnd
ef'lect
caused
by
diflering
pipetting protrciencl,).
(d)
Place tips on
the
12-channel
pipetlol
and
transfèr
100
¡rL
controls and
sample
extracts
to
fhe
antibodv-coated
lells.
Mix
for
20
s
b¡'
sliding
the
well
holder
back and
forth
on
a
lìat
surlàce.
(e)
Incubate
¡licrouells
Table
9.
Lot-to-lot variability
Lot
No.
Lot-to-Loi
1
59002
6/8/1
1
159005
9130/11
159008
11t27111
I0
r¡
in
at
ambient
Samples, ppm
Run
1t¡me.
min
10
'10:10:10
8:8.6
12:12.12
8:1O:12
0_00
0_00
0.00
0.50
5.40
5.60
6.35
5.50
10.50
9.60
9.60
L60
Run 2
tìme
min
0,00
5.00
10.00
10:10:10
8:8:8
12:12.12
8:10
12
0.20
0.00
0.00
0_00
4.90
5.30
5.1
0
5.40
10.40
10.10
9.90
900
Level,
ppm
l2daystoexpiry.
124daystoexpiry.
181
daystoexpiry,
ppm
ppm
ppm
0
0
0
5
10
10
10
0.00
0.00
0.00
4.60
4,80
5,10
9.70
10.10
9.70
0.995
0.00
0.00
0.10
4.80
5.20
5_40
10.50
9.90
10.20
0.994
0.00
0.50
0.00
4.70
4.60
4.50
10.40
10.2Q
10.20
0.985