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Existing methods
LC/MS/MS method to target allergen peptides
Allergen
protein
Digest
Peptides
Peptide - Q1 m/z
200 400 600 800
m/z
0
Peptide - MS/MS m/z
*
*
*
*
Extract
Analysis
Identification
Sample Preparation
LC Ionization MS/MS (MRM mode)
Screening
Existing LC/MS/MS Methods
•
Revision of the sample
Extract proteins from
food sample (1 hour in
preparation utilizing
automated online SPE
•
Total sample preparation
time down to 4.5 hours
•
Results achieved through
modification:
•
Prep‐time reduced by 4x
buffer)
Centrifuge and collect
extract (30 min)
Reduce, alkylate, and
digest with trypsin (
2.5
hours
)
•
Sensitivity improved by 2‐4x
•
LC‐MS/MS run time
reduced by nearly 2x
•
Addition of more allergens
(milk, egg, peanut, treenuts,
gluten, sesame, mustard)
Filter digested extract
(15 min)
Perform automated
online SPE clean-up (
0
hours hands-on time
)
LC-MS/MS analysis