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Regulatory Guidance / Issues
•
Food Allergen Labeling and Consumer Protection Act of
2004.
–
Labeling requires the word “Contains” followed by the name of the
major food allergen – for example, “Contains milk, wheat” – or a
parenthetical statement in the list of ingredients – for example,
“albumin (egg)”
•
Regulation (EU) No 1169/2011
–
on the provision of food information to consumers entered into
application on
13 December 2014
. The obligation to provide
nutrition information will apply from
13 December 2016
.
Fitness for Purpose
XICof+MRM(71pairs):Exp1 929986/1116601DaID:[e]ELINSWVESQTNGIIRfromSample1(E100mrmepi4allergen1612081)ofE100mrme
Max 71000cps
,
.
.
...
.
.
.
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time,min
0.0
5000.0
1.0e4
1.5e4
2.0e4
2.5e4
2.8e4
Intensity,cps
12.69
XIC of+MRM (71 pairs):Exp 1, 688.830/833.400 DaID: [p]DLAFPGSGEQVEK fromSample 1 (P100mrmepi4 allergen 161208 1) ofP100mrmepi4 ...
Max. 9900.0 cps.
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time min
0.0
5.0e4
1.0e5
1.5e5
2.0e5
2.5e5
3.0e5
Intensity, cps
9.67
7.27
Egg peptides
Peanut peptides
•
Benefits of LC‐MS/MS:
–
Improved throughput
through multiplexing
–
Multiple allergens in
one injection
–
Multiple fragments for
each allergen detected
,
XIC of+MRM (71 pairs):Exp 1, 684.350/1183.570 DaID: [m]ALNEINQFYQK fromSample 1 (M100mrmepi4 allergen 161208 1) ofM100mrmepi4 all...
Max. 7.7e4 cps.
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0 11.0 12.0 13.0 14.0 15.0 16.0
Time,min
0.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
7.0e5
8.0e5
8.8e5
Intensity,cps
10.08
Milk peptides
–
More confidence for
fewer false positive or
false negative results
Multi-allergen analysis