Question 1
In your experience using the method, does the method perform according to the method’s applicability
Comment
1
2
3
4
5
6
7
8
Question 2
In your experience with the method, are there any safety concerns identified while using or regarding u
Comment
1
2
No. The hydrolysis temperature written in the method had to be changed before the data for the
phosphatidylinositol practice sample was accepted. In addition, the recovery for the free myo-inositol
for certain matrices were affected by changes in the sample size.
The sample cleanup for phosphatidylinositol determinations needs to be performed in a fume hood. 2)
After hydrolysis, 50% NaOH needs to be added very slowly to avoid splash of the solution, or use more
diluted NaOH solution for the neutralization.
User Comments
NO
User Comments
No for phosphatidylinositol determination: the protocol of extraction and sample clean up is well
described but very tricky. We didn’t manage to analyze the SRM1849a (no pik). In a lab for routine
analysis it will be impossible to perform this test. If it is of interest another way should be use.
Paragraph D. Procedure / 3. HPLC analysis/ Instrument start up: In our opinion it’s necessary to use
helium to pressurize mobile phase. In our opinion, “Area where temperature fluctuations will be
minimal throughout the run” is not precise enough. The oven temperature we use for the HPLC column
is 30°C and the resolution is much better.
Paragraph C. Preparation of reagent: Mobile phase Expiration 1 month seems to be very long (the
temperature of storage is not indicated). Solutions can be stored at 2-30°C(very large?)
Yes
It is change in part of the IC instrument in PAD setting base on MLT organizer
Change in step heat of bound-inositol from 110 to 130 C base on MLT organizer
Yes
