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Question 3
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Question 4
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Question 5
Do you have any general comments about the method?
Comment
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Need change Base on comment in no. 1
Set a guidance on how to determine the appropriate hydrolysis temperature to ensure complete
hydrolysis.
Please see the answer to Q2.
No other safety concerns that were not addressed in the written method.
Yes, the sample prep for the Phosphatidylinositol Determinations: section C. Hydrolysis, 3) the addition
of 50% NaOH. I did not find a way to add the 50% NaOH, that would not cause a violate reaction. Was
afraid sample of sample lose or may blow over.
Shoud work in the fume hood and ware personel protective equipment(PPE), For Chloroform should
add information that it is“ suspected of causing cancer” to be more awearness during using.
This method required an important instrumentation (2 pumps, a switching valve, 2 CarboPac
Columns…). We experiment a gradient and the resolution is as good as with this system. Is it
necessary to include in the method the quantification of myo-inositol bound as phosphatidylinositol ?
(as we didn’t manage to perform this analysis we don’t know if it is of interest).
I really like your column switching and I'm looking at possibly modifying our method to use this for
other types of products other than infant formula. We analyze all sorts of matrices from clients that
contain a lot of things that stick really well to the MA1 column and even require blank injections after
to remove anything remaining. The other good thing is this stuff doesn't see the electrode as well
when you divert it away
Recomment standard to use incase need to prove the %Recovery of boun-inositol analysis need
additional add for the SPE step “
All diluent should rinse into centrifuge tube before rinse through the
SPE.
Need additional guid for Free inositol in step of adjustment to pH 4.5 “To determine the amount
of acid that should be added, transfer small amont of sample solution into a small beaker, and insert a
pH electrode into the beaker. Add 0.5% hydrochloric acid in 0.5 mL increments until the sample pH is
4.5. Record the amount of acid adde.
The column-switching technique was a good idea for reducing the run time.
Do you have any information regarding the reproducibility of this method as written? If so, please spec
No more data for the reporducibility because for bound inositol the temperature in step heat is just
final aglingment to use 130C in May 2014, instead of 110 or 120C.
Based on your experience with the method, are there any recommended changes to the AOAC First Act
None.
User Comments
No
