97 / 166
Chicken Egg
93
•
NIST 8445 (Spray dried whole
egg
for allergen detection)
94
95
9.8.
Validation Guidance
:
96
97
Method developers
should must
provide data for method performance in all the claimed
98
matrixesmatrices.
99
100
Method developers must provide recovery data using incurred samples for all claimed
101
matrices.
102
103
Add appropriate Appendix reference for PTM requirement (Appendix J)
104
105
Appendix D :Guidelines for Collaborative Study Procedures To Validate Characteristics of a
106
Method of Analysis
;
19
th
Edition of the AOAC INTERNATIONAL Official Methods of Analysis
107
(2012). Available at:
http://www.eoma.aoac.org/app_d.pdf108
109
Appendix F :Guidelines for Standard Method Performance Requirements; 19
th
Edition of the
110
AOAC INTERNATIONAL Official Methods of Analysis (2012). Available at:
111
http://www.eoma.aoac.org/app_f.pdf112
113
Appendix M: Validation Procedures for Quantitative Food Allergen ELISA Methods:
114
Community Guidance and Best Practices; 19
th
Edition of the AOAC INTERNATIONAL Official
115
Methods of Analysis (2012). Available at:
http://www.eoma.aoac.org/app_m.pdf116
117
118
Table 1: Method performance requirements
(deleted wine section)
119
120
Parameter
Minimum Acceptance Criteria
for target
matrix
Cookies, Bread, Dough, Salad Dressing
Analytical Range
(ppm)
≤<
5
-
≥>
10
LOQ
(ppm)
≤5
LOD (ppm)
≤5
Recovery (%)*
50-150%
% RSD
r
≤20 %