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Single Laboratory Validation – AOAC First Action Method 2012.22 Vitamin C in Infant Formula and Adult/Pediatric
Nutritional Formula by UPLC-UV
Date of Issue: February 14
th
, 2013
RDLS-RD130018
E.Campos Giménez (NRC/QS, Lausanne)
Page 9
Conclusions and next steps
Conclusions
Method performance characteristics met Standard Method Performance
Requirements (SMPR AOAC 2012.012) for all the matrices in the scope of the
project. Accuracy of the method was proven by analysis of a Certified Reference
Material (SRM 1849a), as well as by recovery rates within 90-110 %. The
precision of the method showed Relative Standard Deviation of repeatability
SDr ≤ 2.5, and Relative Standard Deviation of intermediate reproducibility
SDiR ≤ 5.5 %.
The results prove that the method is a good candidate for further validation by
Multi Laboratory Testing in order to grant Final Action status.
Next steps
Next step is to report SLV data to AOAC for revision by the ERP and publication
of the method in the J. AOAC Int.
If the method is accepted to pursue further validation, a Multi Laboratory Testing
will take place. E. Campos Giménez will be Study Director.
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VitC-03/2012.22
For ERP Use Only
DO NOT DISTRIBUTE
AOAC OMB Meeting Book
319