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Single Laboratory Validation – AOAC First Action Method 2012.22 Vitamin C in Infant Formula and Adult/Pediatric
Nutritional Formula by UPLC-UV
Date of Issue: February 14
th
, 2013
RDLS-RD130018
E.Campos Giménez (NRC/QS, Lausanne)
Page 8
Single Laboratory Validation,
Continued
Accuracy
studies
Reference material
: The overall mean of duplicate analysis of the certified
reference material SRM 1849a on 6 different days by 2 analysts was
70.3 mg/100 g, with a SD (standard deviation of the mean of duplicates) of 2.9
mg/100 g, which is not statistically different from the certified value (78.4 ± 6.5
g/100 g).
Spike recovery:
Results of spiking experiments are shown below. All recovery
rates complied with requirements (90-110 %).
Target +50 % of target + 100 % of target
Non fortified products
Avg
RSD
Avg
RSD
Child Formula Powder
4.4
111 % 0.9 %
98 %
1.8 %
Infant Elemental Powder
40
93 %
0.9 %
94 %
1.1 %
Adult Nutritional RTF High Prot
30
97 %
2.4 %
93 %
2.0 %
Adult nutritional RTF High Fat
35
98 %
1.1 %
95 %
2.3 %
Infant Formula RTF Milk Based
10
99 %
0.6 %
97 %
1.4 %
Example
chromatogra
ms
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
0.045
0.050
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Standard solutionAscorbic acid2 µg/mL
Infant FormulaPowder Milk Based
Infant FormulaPowder Soy Based
SRM 1849a
Ascorbic
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Ascorbic
AU
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
Minutes
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
Infant Elemental Powder
Adult Nutritional RTF HighFat
9 / 20
VitC-03/2012.22
For ERP Use Only
DO NOT DISTRIBUTE
AOAC OMB Meeting Book
318