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Single Laboratory Validation – AOAC First Action Method 2012.22 Vitamin C in Infant Formula and Adult/Pediatric
Nutritional Formula by UPLC-UV
Date of Issue: February 14
th
, 2013
RDLS-RD130018
E.Campos Giménez (NRC/QS, Lausanne)
Page 7
Single Laboratory Validation,
Continued
LoD/LoQ
Results from 10 independent analysis of a placebo sample spiked at low
concentrations are given below:
Infant Formula RTF, milk based
Average
(mg/100 g)
RSD
Accuracy
Spike level 2.5 mg/100 g
2.47
1 %
99 %
Spike level 1.25 mg/100 g
1.29
1 %
103 %
Spike level 0.5 mg/100 g
0.65
3 %
130 %
Although the precision at 0.5 mg/100 g was still acceptable, the accuracy did not
meet the performance requirements. Thus, the quantification limit was set at
1.25 mg/100 g.
Precision
studies
Results of precision studies are shown below.
Requirements (SMPR)
≤ 5 %
Mean
(mg/100 g)
RSD
r
(%)
RSD
iR
(%)
SRM 1849a
7.0
2.0
4.4
Adult Nutritional Powder Milk Protein Based
5.9
1.0
3.9
Infant Formula Powder Part Hyd Milk Based
13.4
0.9
5.5
Infant Formula Powder Part Hyd Soy Based
19.1
0.9
2.6
Adult Nutritional Powder Low Fat
13.5
1.3
3.1
Child Formula Powder
4.8
0.5
1.0
Infant Elemental Powder
36.8
1.7
1.3
Infant Formula Powder Milk Based
11.8
1.4
1.4
Infant Formula Powder Soy Based
10.6
1.4
2.0
Infant Formula RTF Milk Based
8.0
0.7
4.8
Adult Nutritional RTF High Protein
29.9
0.8
4.2
Adult Nutritional RTF High Fat
39.3
2.5
5.3
RSD
r
is the relative standard deviation of repeatability
RSD
iR
is the relative standard deviation of intermediate reproducibility
Continued on next page
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VitC-03/2012.22
For ERP Use Only
DO NOT DISTRIBUTE
AOAC OMB Meeting Book
317