337 / 435
Single Laboratory Validation – AOAC First Action Method 2012.22 Vitamin C in Infant Formula and Adult/Pediatric
Nutritional Formula by UPLC-UV
Date of Issue: February 14
th
, 2013
RDLS-RD130018
E.Campos Giménez (NRC/QS, Lausanne)
Page 2
Introduction
Project
background
Infant formula industry has defined a strong need to modernize and harmonize
analytical standards to verify compliance of infant/adult nutritionals. It is
important that these standards are accepted and applied worldwide to ensure
fare trade, benefit inter-market supply and dispute resolution.
The objective of this project is to establish international consensus methods for
infant formula and adult nutritionals. This will benefit inter-market supply and
dispute resolution. AOAC, mandated by the Infant Formula Council, can
provide the scientific tools to accomplish this by offering a forum to bring
together all stakeholders and reach consensus on the requirements of these
future standards.
Under this context, a new path to achieve AOAC Official Method status was
approved in 2011
(1)
. According to this alternative path, methods proposed and
reviewed within dedicated AOAC Working Groups can be granted Official First
Action status by vetted Expert Review Panels (ERP). The methods remain First
Action for a period of about 2 years. During this time, information on method
performance generated through the use of the method is monitored. The
performance of the method is then reviewed by the ERPs to determine whether
Final Action should be recommended to the Official Methods Board. As an
intermediate stage to achieve final AOAC Official Methods of Analysis, First
Action Method Status was approved in October, 2012
Vitamin C
method status
Based on the data presented in a previous publication and on a Single
Laboratory Validation study on a selection of matrices previously reported
(2,3)
,
the method “Vitamin C in Infant Formula and Adult/Pediatric Nutritional Formula
by UPLC-UV” proposed by Nestlé was granted First Action status and
designated AOAC Method 2012.22
(4)
on October 2012. The method
corresponds to LI-00.651 “Vitamin C by HPLC-UV”, with adaptation on sample
preparation to decrease amount of chemicals, optimize protein precipitation and
increase speed.
Next steps included completion of SLV data following SPIFAN guidelines
(Attachment 1) on a complete set of SPIFAN matrices (Attachment 2). The data
generated will be reviewed by the ERP in March 2013.
The work presented herein summarizes the full Single Laboratory Validation on
SPIFAN matrices.
3 / 20
VitC-03/2012.22
For ERP Use Only
DO NOT DISTRIBUTE
AOAC OMB Meeting Book
312