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REVISED 121213
caps, 8 caps per strip, 12 strips per kit; lysis buffer, 1 bottle, 60 mL; lysis reagent, 3 vials, lyophilized; kit
1
insert. Store reagent tubes at 2-8
o
C, in sealed foil pouches with desiccant. Store lysis buffer at 2-8
o
C.
2
(b)
Phosphate-buffered saline
. – Per liter: 8.0 g NaCl, 0.2 g KCl, 1.44 g Na
2
HPO
4
, 0.24 g KH
2
PO
4
.
3
(c)
Hektoen enteric agar
. – Available from Neogen Corp. and other suppliers. Follow
4
manufacturer’s instructions for preparation.
5
(d)
Xylose lysine deoxycholate (XLD) agar
. – Available from Neogen Corp. and other suppliers.
6
Follow manufacturer’s instructions for preparation.
7
(e)
Bismuth sulfite agar
. – Available from Neogen Corp. and other suppliers. Follow manufacturer’s
8
instructions for preparation.
9
(f)
Brilliant green sulfa agar
. – Available from Neogen Corp. and other suppliers. Follow
10
manufacturer’s instructions for preparation.
11
(g)
Xylose lysine tergitol (XLT-4) agar
. – Available from Neogen Corp. and other suppliers. Follow
12
manufacturer’s instructions for preparation.
13
(h)
Double-modified lysine iron agar
. – Available from various suppliers. Follow manufacturer’s
14
instructions for preparation.
15
(i)
Tryptic soy agar
. – Available from Neogen Corp. and other suppliers. Follow manufacturer’s
16
instructions for preparation.
17
C.
Apparatus
18
(a)
Incubator/reader
. – Available from Neogen Corp. Incubator/reader capable of operating at 56 ±
19
1
o
C and reading fluorescence in real time in 2 channels (485/535 nm and 540/590 nm).
20
(b)
Computer and ANSR software
. – Available from Neogen Corp. For connection to
21
incubator/reader. Minimum requirements for computer: Intel® Core i3 processor, 1 GB RAM,
22
Windows® 7, Ethernet and USB connections.
23
(c)
Heater block
. – With insert for 1.2 mL cluster tubes, 80 ± 2
o
C.
24
(d)
Micropipettor
. – 50 µL, fixed or adjustable volume.
25
6