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SUBMITTED 050113, REVISED 102513
(g)
Xylose lysine tergitol (XLT-4) agar
. – Available from Neogen Corp. and other suppliers. Follow
1
manufacturer’s instructions for preparation.
2
(h)
Double-modified lysine iron agar
. – Available from various suppliers. Follow manufacturer’s
3
instructions for preparation.
4
(i)
Tryptic soy agar
. – Available from Neogen Corp. and other suppliers. Follow manufacturer’s
5
instructions for preparation.
6
7
C.
Apparatus
8
(a)
Incubator/reader
. – Available from Neogen Corp. Incubator/reader capable of operating at 56 ±
9
1
o
C and reading fluorescence in real time in 2 channels (485/535 nm and 540/590 nm).
10
(b)
Computer and ANSR software
. – Available from Neogen Corp. For connection to
11
incubator/reader. Minimum requirements for computer: Intel® Core i3 processor, 1 GB RAM,
12
Windows® 7, Ethernet and USB connections.
13
(c)
Heater block
. – With insert for 1.2 mL cluster tubes, 80 ± 2
o
C.
14
(d)
Micropipettor
. – 50 µL, fixed or adjustable volume.
15
(e)
Pipettor
. – 100-1,000 µL, adjustable volume.
16
(f)
8-channel micropipettor
. – 50 µL, fixed or adjustable volume.
17
(g)
Pipette tips
. – 50 µL, with filter.
18
(h)
Pipette tips
. – 1,000 µL.
19
(i)
Tubes.
– Glass or plastic, 12 × 75 mm or similar, sterile, with caps.
20
(j)
Inoculating loops or needles
. – Sterile.
21
22
D.
Preparation of Test Samples
23
27