1109 / 1195
SUBMITTED 050113, REVISED 102513
The assay detects serovars of both
S. enterica
and
S. bongori
, including all genetic subgroups. In testing
1
of 113 strains of
Salmonella
spp., representing 109 serovars, only a single strain of
S
. Weslaco was not
2
detected.
3
4
Study Design
5
6
Experimental Design
7
The study is designed to determine the ability of the ANSR
Salmonella
assay to correctly identify
8
colonies of known
Salmonella
spp. and non-salmonellae taken from common selective/differential agar
9
media as “
Salmonella
spp.” or “not
Salmonella
spp.”. The study was conducted in accordance with the
10
AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food
11
and Environmental Surfaces
, Appendix J [6], with particular reference to section 6, Confirmatory
12
Identification Methods. Biosafety Level 2 procedures were employed in the conduct of this study.
13
14
Test Strains
15
Test strains were obtained from the American Type Culture Collection (ATCC, Manassas, VA), the U.S.
16
Centers for Disease Control and Prevention (CDC; Atlanta, GA), and other sources.
17
18
The inclusivity panel contained 113 strains representing
S. enterica
and
S. bongori
, all genetic subgroups,
19
and 108 different serovars including somatic groups A through Z and 51, 53, 55, 56, 57, 59, 60, 66, and
20
67. The majority of the strains (85%) are from the CDC collection. These strains have been maintained
21
in -80
o
C storage at GENE-TRAK Systems and Neogen Corp. and have been used in several previous AOAC
22
studies. Inclusive strains are shown in Table 1.
23
24
The exclusivity panel contains 37 strains representing 15 genera and 36 species. All but 5 strains were
25
obtained directly from ATCC. As with the inclusivity panel, these strains have been maintained in -80
o
C
26
storage and have been used in several prior AOAC studies. The exclusive strains are shown in Table 2.
27
28
Growth of Test Strains
29
The inclusive and exclusive strains were randomized, blind coded, and divided into blocks for testing as
30
follows. 112 inclusive strains were listed in an Excel spreadsheet followed by 38 exclusive strains. A
31
30