SUBMITTED 050113, REVISED 102513
The assay detects serovars of both
S. enterica
and
S. bongori
, including all genetic subgroups. In testing
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of 113 strains of
Salmonella
spp., representing 109 serovars, only a single strain of
S
. Weslaco was not
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detected.
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Study Design
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Experimental Design
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The study is designed to determine the ability of the ANSR
Salmonella
assay to correctly identify
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colonies of known
Salmonella
spp. and non-salmonellae taken from common selective/differential agar
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media as “
Salmonella
spp.” or “not
Salmonella
spp.”. The study was conducted in accordance with the
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AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food
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and Environmental Surfaces
, Appendix J [6], with particular reference to section 6, Confirmatory
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Identification Methods. Biosafety Level 2 procedures were employed in the conduct of this study.
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Test Strains
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Test strains were obtained from the American Type Culture Collection (ATCC, Manassas, VA), the U.S.
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Centers for Disease Control and Prevention (CDC; Atlanta, GA), and other sources.
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The inclusivity panel contained 113 strains representing
S. enterica
and
S. bongori
, all genetic subgroups,
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and 108 different serovars including somatic groups A through Z and 51, 53, 55, 56, 57, 59, 60, 66, and
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67. The majority of the strains (85%) are from the CDC collection. These strains have been maintained
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in -80
o
C storage at GENE-TRAK Systems and Neogen Corp. and have been used in several previous AOAC
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studies. Inclusive strains are shown in Table 1.
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The exclusivity panel contains 37 strains representing 15 genera and 36 species. All but 5 strains were
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obtained directly from ATCC. As with the inclusivity panel, these strains have been maintained in -80
o
C
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storage and have been used in several prior AOAC studies. The exclusive strains are shown in Table 2.
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Growth of Test Strains
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The inclusive and exclusive strains were randomized, blind coded, and divided into blocks for testing as
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follows. 112 inclusive strains were listed in an Excel spreadsheet followed by 38 exclusive strains. A
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