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DuPont™ BAX

®

System Real-Time PCR Assay for

Salmonella

: Collaborative Study

DuPont Nutrition & Health

Page 28

Acknowledgements

1

Collaborators included:

2

Bryan Dieckelman and Tom Donohue, Deibel Laboratories; Hesham Elgaali Indiana Department of

3

Health; Willis Fedio, New Mexico State University; Elizabeth Galbraith, DuPont Nutrition & Health; Brian

4

Kupski, Silliker Laboratories; Kristina McCallum and Dominika Kondratko, Colorado Department of

5

Agriculture; Guy McWhorter, ABC Research Laboratories; Joe Meyer and Demi Swift, Covance

6

Laboratories; Roy Radcliff and Dorn Clark, Marshfield Food Safety; Dora Rodgers, Kate Angeles and

7

Joseph Putrow, Wisconsin Department of Agriculture; and Leslie Thompson, AEGIS FOOD TESTING

8

Laboratories.

9

The authors would also like to thank Nicolette Blubaugh for invaluable assistance in the preparation of

10

this manuscript and Maria Nelson for her expertise and assistance in the design and execution of this

11

study.

12

References

13

1.

U.S. Department of Agriculture-Food Safety and Inspection Service

Microbiological Laboratory

14

Guidebook

Chapter 4.05 (2011),

http://www.fsis.usda.gov/PDF/MLG_4_05.pdf

15

2.

U.S. Food and Drug Administration

Bacteriological Analytical Manual

, Chapter 5 (2011),

16

http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManu

17

alBAM/ucm070149.htm

18

3.

Reid, A. MFHPB-20, Isolation and Identification of

Salmonella

from Food and Environmental

19

Samples. 2009 In: Health Canada Compendium, Vol. 3, Laboratory Procedures for the

20

Microbiological Examination of Foods. Health Canada, Health Products and Food Branch,

21

http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/volume2/mfhpb20-01-eng.php

22

4.

Brunelle, S., LaBudde, R., Nelson, M, and Wehling, P. (2011) AOAC INTERNATIONAL Methods

23

Committee Guidelines for Validation of Microbiological Methods for Food and Environmental

24

Surfaces

Appendix X

25

5.

Least Cost Formulations, Ltd. , MPN Calculator-Version 1.6

26

(http://www.lcfltd.com/customer/LCFMPNCalculator.exe)

27

6.

Least Cost Formulations, Ltd. , AOAC Binary Data Interlaboratory Study Workbook (2011)

28

(http://lcfltd.com/aoac/aoac-binary-v2-2.xls

)

29

FINAL (Version 4)