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Detection of
Salmonella
species in a Variety of Foods by the
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DuPont™ BAX
®
System Real-Time PCR Assay for
Salmonella
:
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Pre-Collaborative Study
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F. Morgan Wallace, Bridget Andaloro, Dawn Fallon, Nisha Corrigan, Stephen Varkey, Daniel DeMarco,
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Andrew Farnum, Monica Tadler, Steven Hoelzer, Julie Weller, Eugene Davis, Jeffrey Rohrbeck, and
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George Tice
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DuPont Nutrition & Health, ESL Building 400, Route 141 & Henry Clay Road, Wilmington, Delaware
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19880
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Patrick Bird, Erin Crowley
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Q Laboratories, Inc., 1400 Harrison Avenue, Cincinnati, Ohio 45214
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A single-laboratory study was conducted to evaluate the ability of the DuPont™ BAX® System Real-
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Time PCR Assay for
Salmonella
to detect the target species in a variety of foods and environmental
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surfaces. Internal validation studies were performed by DuPont Nutrition & Health on 18 different
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sample types to demonstrate the reliability of the test method among a wide variety of sample types.
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Most sample types tested in this study were artificially contaminated with a
Salmonella
strain at
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levels expected to produce low (0.2–2.0 cfu/test portion) or high (5 cfu/test portion) spike levels on
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the day of analysis; one sample type (chicken wings) was naturally contaminated. The results of the
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pre-collaborative study demonstrate that there is no statistically significant difference in performance
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between the BAX® System method and the reference methods for detection of
Salmonella
for the
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sample types evaluated.
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Introduction
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Salmonella
is a leading cause of foodborne illness. The low infectious dose of the bacterium makes it
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critical to detect even low concentrations of the
Salmonella
in foods. Additionally, the presence of high
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concentrations of closely related non-pathogenic bacteria create the need for highly accurate
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methodologies. Traditionally, labs concerned with detection of
Salmonella
screened food samples with
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culture methods, such as those provided by the United States Department of Agriculture Food Safety
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and Inspection Service (USDA-FSIS) and the U. S. Food and Drug Administration’s (FDA), which require
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several days to detect and confirm
Salmonella
. Rapid methods of screening for
Salmonella
have been
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developed, but these generally require two days of enrichment. By contrast, the DuPont™ BAX® System
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detects the pathogen less than 90 minutes after enrichment, and the DNA-based results are both
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reliable and reproducible, leading to quicker release of cleared product.
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Dupont BAX Salmonella Pre- Collaborative Study
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Do Not Distribute
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