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AOAC INTERNATIONAL
Place a PCR tube holder on the PCR cooling block. Insert one PCR tube per sample into the
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holder and remove caps. Using a multi-channel pipette, transfer 30
µ
L sample lysate to a PCR
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tube. Cap PCR with the flat optical cap strips provided in the kit.
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Follow screen prompts to load samples into the cycler/detector and begin the program. At the
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completion of the PCR and detection process, follow the screen prompts to remove samples and
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display results.
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The results are recorded on the rack display or from a spreadsheet printout of the results (called
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Detail View). Negative results are indicated by the green circle with (-) symbol, positive results are
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indicated by the red circle with (+) symbol, and indeterminate results are indicated with a yellow
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circle with (?) symbol. A yellow circle with a (?) symbol and a red slash indicated a low signal or
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signal error.
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Statistical Analysis was conducted as described in the AOAC INTERNATIONAL Methods
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Committee Guidelines for Validation of Microbiological Methods for Food and Environmental
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Surfaces [5] using the POD approach. Additionally, a Chi Square statistical analysis was
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performed. Unless otherwise stated, all BAX® System testing was performed with and without a
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regrowth step (10 uL transferred to 500 uL of pre-warmed BHI broth and incubated for 3 hrs at
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37°C) and results were identical with and without this step. The exception was the dry pet food, in
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which a regrowth was required from both enrichment methods due to PCR inhibitory compounds
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in the primary enrichment broths.
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Dupont BAX Salmonella PTM Report
PTM Certification No. 081201 (08/07/2012)
For Expert Review Panel Use Only
Do Not Distribute