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3M Molecular Detection Assay Salmonella Collaborative Study
July 2012
OMA-2012-July-XXX
Appendix 8.4
1
Study Materials
2
3
Materials and reagents provided by the study sponsor
4
5
1.
3M Molecular Detection kits
6
2.
3M Molecular Detection Instrument, power supply, cord, USB cable and software
7
3.
3M Molecular Detection Speed Loader Tray
8
4.
3M Molecular Detection Chill Block Tray and Chill block insert
9
5.
3M Molecular Detection Heat Block Insert
10
6.
3M Molecular Detection Cap/Decap Tool [Reagent]
11
7.
3M Molecular Detection Cap/Decap Tool [Lysis]
12
8.
Empty lysis tube rack
13
9.
Empty reagent tube rack
14
10. Sterile filter-tip pipette tips – capable of 20µL
15
11. Filter Stomacher® bags – Seward or equivalent
16
17
Enrichment
18
12. Lactose broth
19
13.
3M BPW ISO
20
14. RV broth
21
15. mRV (Hanja formulation)
22
16. TT broth
23
24
Selective agar
25
17. Hektoen Agar
26
18. XLD Agar
27
19. Bismuth Sulfite
28
20. XLT4 Agar
29
21. Brilliant green sulfa agar
30
31
Confirmation
32
22. TSI
33
23. LIA
34
24. API 20E or VITEK
35
25. O and H serotyping supplies
36
37
38
Additional Supplies and Reagents required but not provided
39
1.
Pipettes – capable of 20µL
40
2.
Multi-channel (8-channel) pipette – capable of 20µL
41
3.
Stomacher® – Seward or equivalent
42
4.
Thermometer – calibrated range to include 100±1°C range
43
5.
Dry double block heater unit – capable of maintaining 100 + 1°C; OR a water bath -
44
capable of maintaining 100 + 1°C
45
6.
Incubators – capable of maintaining 37 ± 1°C
46
DRAFT DOCUMENT
22