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3M Molecular Detection Assay Salmonella Collaborative Study
July 2012
OMA-2012-July-XXX
Appendix 8.6 – Flow Diagram for Raw Ground Beef
1
2
3
Matrix
0.1ml sample + 10ml mRV
0.5ml sample + 10ml TT (Hanja)
22-24h at 42 ± 0.5°C
• Streak to XLT4 (or DMLIA) & BGS
Incubate at 35±2°C
Examine at 18-24h & 42-48h
Transfer typical colonies to
TSI/LIA slants
24±2h at 35±2°C
25g + 225mLBPW
Homogenize 2 min
20-24h h at 35±2°C
Biochemical/serological
confirmations on all
enrichments
according to
USDA/FSIS
Run the 3M Molecular
Detection Assay
following IFU
Confirmation of
test method
3M MDA
USDA
25g + 225mL 3M BPW ISO
(prewarmed to 37C)
Homogenize 2 min
8 h at 37±1°C
DRAFT DOCUMENT
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