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3M Molecular Detection Assay Salmonella Collaborative Study
July 2012
OMA-2012-July-XXX
Appendix 8.5 - Flow Diagram for Wet Dog Food
1
2
3
Matrix
25g + 225ml Lactose broth,
Blend 2 min and let stand 60±5 min at room temp
Mix and adjust pH to 6.8±0.2 if necessary
24±2h h at 35°C
0.1ml sample + 10ml RV, 24±2h at 42±0.2°C
1.0ml sample + 10ml TT, 24±2h at 35±2°C
Transfer typical colonies
to TSI/LIA slants
24±2h at 35°C
Confirmation of
test method
BAM
Biochemical/serological
confirmation on all
enrichments
according to
FDA-BAM
• Streak to XLD & HE - 24±2h at 35°C
• Streak to BS - incubate at 35°C for
24±2h, read and pick if applicable.
Re-incubate another 24 h
Run the 3M Molecular
Detection System
following IFU
3M MDA
375g + 3.375L 3M
BPW ISO
(prewarmed to 37C)
Homogenize 2 min
18 h at 37±1°C
NOTE - Confirmation of test method
begins with a transfer from the 3M primary
enrichment (BPW) into RV and TT.
DRAFT DOCUMENT
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