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a

% injury calculated as

100 )

1(

x

n

n

nonselect

select

where

n

select

= mean number of colonies on selective agar and

n

nonselect

= mean number of colonies on nonselective agar

1

b

MPN = Most Probable Number is based on a 3-tube, 4-dilution protocol (100g, 10g, 1g, 0.1g)

2

c

X

2

is defined by Mantel-Haenszel as (n-1)(AF-(B+C+D)E)

2

/(A+B+C+D)(A+E)B+C+D+F)(E+F) where n+A+B+C+D+E+F. A X

2

value greater than 3.84 indicates significance at

p

>0.05.

3

4

25