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Figure 1. 3M Molecular Detection Assay
Salmonella
vs USDA MLG (ground beef, breaded chicken nuggets,
1
pasteurized liquid egg)
2
3
Matrix
Screen for
Indigenous
Salmonella
Estimate
total aerobic
counts
0.1ml sample + 10ml mRV
0.5ml sample + 10ml TT (Hanja)
22-24h at 42 ± 0.5°C
• Streak to XLT4 (or DMLIA) & BGS
Incubate at 35±2°C
Examine at 18-24h & 42-48h
Transfer typical colonies to
TSI/LIA slants
24±2h at 35±2°C
Ground beef:
25g + 225mL*
Cooked breaded chicken:
325g + 2.925L*
Liquid egg:
100g + 900 mL*
Homogenize 2 min
*BPW
Biochemical/serological
confirmations on all
enrichments
according to
USDA/FSIS
Run the 3M Molecular
Detection System following
IFU
Confirmation of
test method
MPN
3M
USDA
Ground beef:
25g + 225mL*
Cooked breaded chicken:
325g + 2.925L*
Liquid egg:
100g + 900 mL*
Homogenize 2 min
18-24h h at 37±1°C
*BPW (prewarmed to 37C)
Raw beef
20-24h h at
35±2°C
Cooked chicken,
liquid eggs
18-24h h at
35±2°C
4
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